Zhang Zhi-kai, Yu Dong-zheng, Zhang Jian-hua, Hai Rong, Cai Hong, Wei Jian-chun
Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Apr;24(4):291-5.
To find out the differences between 102 kb pgm locus of Yersinia pestis isolated from Microtus brandti with of other types, and the characters of Yersinia pestis isolated from Microtus brandti caused by their makeup of the 102 kb pgm locus.
102 kb pgm locus of Yersinia pestis isolated from Microtus brandti and Yersinia pestis isolated from Marmota himalayana were amplified by polymerase chain reation (PCR) with 25 pair of nested primers. The PCR products of one pair of primer were obviously different, and then cloned and sequenced. Sequences were searched against current protein and nucleotide databases, using BLAST.
The 102 kb pgm locus of Yersinia pestis isolated from Microtus brandti was devoid one IS100. In addition, it had more copies than other types in the similar variable-number tandem repeat sequences.
The 102 kb pgm locus of Yersinia pestis was different from that of other types. It had only one IS100 flanked it, which corresponded to the character that its pgm(+) phenotype was stable. Further study was needed to confirm the relationship between the diminution virulence of Yersinia pestis isolated from Microtus brandti and the loss of IS100 and other changes.
了解从布氏田鼠分离的鼠疫耶尔森菌102 kb pgm基因座与其他类型的差异，以及由其102 kb pgm基因座组成导致的从布氏田鼠分离的鼠疫耶尔森菌的特征。
用25对巢式引物通过聚合酶链反应（PCR）扩增从布氏田鼠分离的鼠疫耶尔森菌和从喜马拉雅旱獭分离的鼠疫耶尔森菌的102 kb pgm基因座。一对引物的PCR产物明显不同，然后进行克隆和测序。使用BLAST在当前的蛋白质和核苷酸数据库中搜索序列。
从布氏田鼠分离的鼠疫耶尔森菌的102 kb pgm基因座缺少一个IS100。此外，在相似的可变数目串联重复序列中，它比其他类型有更多的拷贝。
鼠疫耶尔森菌的102 kb pgm基因座与其他类型不同。它只有一个IS100位于其侧翼，这与它的pgm(+)表型稳定的特征相对应。需要进一步研究以证实从布氏田鼠分离的鼠疫耶尔森菌毒力减弱与IS100缺失及其他变化之间的关系。
