Thiry M
Laboratory of Cellular and Tissular Biology, University of Liège, Belgium.
Nucleic Acids Res. 1992 Dec 11;20(23):6195-200. doi: 10.1093/nar/20.23.6195.
We have investigated the fine spatial distribution of RNA and rRNA within the Ehrlich tumor cell nucleolus by in situ hybridization with a biotin-labeled probe and by two new strategies, the polyadenylate nucleotidyl transferase-immunogold technique and immuno-labeling with anti-RNA antibodies. Besides the presence, as expected, of RNA and rRNA in the granular component and the dense fibrillar component, we show, for the first time, significant label over all the fibrillar centers of the nucleoli. When RNA and DNA were detected simultaneously on the same sections, only the fibrillar centers were positive for both. These results throw light on the controversial subject of the precise location of transcribing rRNA genes within the nucleolus. The fibrillar centers, and not the dense fibrillar component, should thus be the site of rRNA synthesis.
我们通过用生物素标记的探针进行原位杂交以及两种新方法，即聚腺苷酸核苷酸转移酶 - 免疫金技术和用抗RNA抗体进行免疫标记，研究了艾氏腹水瘤细胞核仁内RNA和rRNA的精细空间分布。除了如预期在颗粒组分和致密纤维组分中存在RNA和rRNA外，我们首次显示核仁所有纤维中心均有明显标记。当在同一切片上同时检测RNA和DNA时，只有纤维中心两者均呈阳性。这些结果阐明了核仁内转录rRNA基因精确定位这一有争议的问题。因此，纤维中心而非致密纤维组分应是rRNA合成的位点。
