Bendayan M, Puvion E
J Ultrastruct Res. 1983 Jun;83(3):274-83. doi: 10.1016/s0022-5320(83)90134-x.
The recently developed RNAase-gold cytochemical method and the more classical high-resolution autoradiography following incorporation of tritiated uridine, were applied for the localization of RNA molecules in thin sections of isolated liver cells cultured under control conditions or submitted to drugs known to alter the distribution of nuclear RNA. The similar pattern of labeling obtained with both techniques under the three experimental conditions studied (control, treatments with CdCl2, or actinomycin D), together with the results obtained after RNAase digestion, are a good indication of the high specificity and sensitivity of the RNAase-gold method and provide a demonstration of the complementarity of these two methods for the study of the ultrastructural distribution of nuclear RNA.
最近开发的核糖核酸酶-金细胞化学方法，以及在掺入氚化尿苷后更经典的高分辨率放射自显影技术，被用于在对照条件下培养的分离肝细胞薄片中定位RNA分子，或应用于已知会改变核RNA分布的药物处理后的肝细胞。在所研究的三种实验条件下（对照、氯化镉处理或放线菌素D处理），两种技术获得的类似标记模式，以及核糖核酸酶消化后得到的结果，充分表明了核糖核酸酶-金方法具有高度的特异性和敏感性，并证明了这两种方法在研究核RNA超微结构分布方面具有互补性。
