Jian Ming, Parker C Richard
Department of Obstetrics and Gynecology, The University of Alabama at Birmingham, Birmingham, AL 35233-7333, USA.
IUBMB Life. 2003 Mar;55(3):155-8. doi: 10.1080/1521654031000106672.
Macrophage migration inhibitory factor (MIF) has been found to be widely expressed in many cell types throughout the body and appears to play several physiologic roles. We sought to determine the expression and cellular distribution of MIF in human fetal (HFA) and adult (HAA) adrenals. A single band of approximately 0.8 kb was revealed by northern blot hybridization using cDNA probes for MIF in total RNA extracts of both HFA and HAA tissues. Immunohistochemical analysis showed strong immunostaining for MIF in the broad fetal zone of the HFA and in both the zona glomerulosa and zona reticularis of HAA. The cells of the zona fasciculata of the HAA and of the neocortex of the HFA were only minimally, if at all, immunopositive for MIF and medullary elements were consistently negative for MIF. These results are indicative of local production of MIF in adrenocortical cells during intrauterine development and also in adulthood. The role of MIF in cortical cells of the human adrenal gland remains to be determined.
巨噬细胞移动抑制因子（MIF）已被发现广泛表达于全身多种细胞类型中，并且似乎发挥着多种生理作用。我们试图确定MIF在人类胎儿肾上腺（HFA）和成人肾上腺（HAA）中的表达及细胞分布。使用针对MIF的cDNA探针，通过Northern印迹杂交在HFA和HAA组织的总RNA提取物中显示出一条约0.8 kb的单带。免疫组织化学分析显示，MIF在HFA的宽阔胎儿带以及HAA的球状带和网状带中均有强免疫染色。HAA束状带的细胞和HFA新皮质的细胞对MIF的免疫阳性反应即使有也极为微弱，而髓质成分对MIF始终呈阴性。这些结果表明，在子宫内发育期间以及成年期，肾上腺皮质细胞中均可局部产生MIF。MIF在人类肾上腺皮质细胞中的作用仍有待确定。
