Xia Harry Hua-Xiang, Lam Shiu-Kum, Huang Xiao-Ru, Wong Wai-Man, Leung Suet-Yi, Yuen Siu-Tsan, Lan Hui-Yao, Wong Benjamin Chun-Yu
Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, S.A.R., China.
J Infect Dis. 2004 Jul 15;190(2):293-302. doi: 10.1086/421915. Epub 2004 Jun 21.
The macrophage migratory inhibitory factor (MIF) plays a pivotal role in inflammatory and immune diseases; however, its role in gastrointestinal diseases has not been clarified. This study intended to determine the expression of MIF, by gastric epithelial cells, T cells, and macrophages, in Helicobacter pylori-induced gastritis. Sixty-four patients (30 males, 34 females; mean age, 47 years) referred for upper endoscopy were recruited. Biopsy specimens from the gastric antrum and corpus were obtained for (1) detection of H. pylori and histological examination, (2) single and double immunostaining to test for expression of MIF protein in epithelial cells, T cells, and macrophages, and (2) in situ hybridization for expression of MIF mRNA within the lamina propria. In mucosal specimens from each of the 2 sites, both the percentage of MIF(+) epithelial cells and the numbers of MIF mRNA(+) inflammatory cells, MIF(+) T cells, and MIF(+) macrophages were significantly higher in H. pylori-positive patients than in H. pylori-negative patients. Overall, the percentage of MIF(+) epithelial cells and the numbers of MIF mRNA(+) cells, MIF(+) T cells, and MIF(+) macrophages were higher in the antrum than in the corpus. The percentage of MIF(+) epithelial cells and the numbers of MIF mRNA(+) cells, MIF(+) T cells, and MIF(+) macrophages increased in chronic gastritis, but, in the absence of H. pylori infection, this increase disappeared for all except MIF(+) T cells. Therefore, H. pylori infection is associated with increased expression of the MIF protein and MIF mRNA in gastric epithelial and inflammatory cells; along with other cytokines, MIF may play a significant role in gastric inflammation related to H. pylori infection.
巨噬细胞移动抑制因子（MIF）在炎症和免疫疾病中起关键作用；然而，其在胃肠道疾病中的作用尚未明确。本研究旨在确定胃上皮细胞、T细胞和巨噬细胞中MIF在幽门螺杆菌诱导的胃炎中的表达情况。招募了64例因上消化道内镜检查前来就诊的患者（男性30例，女性34例；平均年龄47岁）。获取胃窦和胃体的活检标本用于：（1）检测幽门螺杆菌及组织学检查；（2）单重和双重免疫染色以检测上皮细胞、T细胞和巨噬细胞中MIF蛋白的表达；（3）原位杂交检测固有层中MIF mRNA的表达。在2个部位的黏膜标本中，幽门螺杆菌阳性患者的MIF(+)上皮细胞百分比以及MIF mRNA(+)炎性细胞、MIF(+) T细胞和MIF(+)巨噬细胞的数量均显著高于幽门螺杆菌阴性患者。总体而言，胃窦中MIF(+)上皮细胞百分比以及MIF mRNA(+)细胞、MIF(+) T细胞和MIF(+)巨噬细胞的数量高于胃体。慢性胃炎中MIF(+)上皮细胞百分比以及MIF mRNA(+)细胞、MIF(+) T细胞和MIF(+)巨噬细胞的数量增加，但在无幽门螺杆菌感染时，除MIF(+) T细胞外，其他各项的增加均消失。因此，幽门螺杆菌感染与胃上皮细胞和炎性细胞中MIF蛋白及MIF mRNA表达增加有关；与其他细胞因子一起，MIF可能在幽门螺杆菌感染相关的胃炎症中起重要作用。
