亚洲玉米螟（Chilo partellus（Swinhoe））（鳞翅目：螟蛾科）对两个玉米（Zea mays L.）品种叶片天然产物的产卵反应

Ovipositional responses of Chilo partellus (Swinhoe) (Lepidoptera: pyralidae) to natural products from leaves of two maize (Zea mays L.) Cultivars.

作者信息

Varshney Anupam K, Babu B Ravindra, Singh Ashok K, Agarwal Hari C, Jain Subhash C

机构信息

Departments of Zoology and Chemistry, University of Delhi, Delhi-110 007, India.

出版信息

J Agric Food Chem. 2003 Jul 2;51(14):4008-12. doi: 10.1021/jf0211074.

DOI:10.1021/jf0211074

PMID:12822938

Abstract

Ovipositional responses of Chilo partellus (Swinhoe) (Lepidoptera: Pyralidae) to hexane extracts of leaves of two maize (Zea mays L.) cultivars, one resistant (Kisan) and one susceptible (Basilocal), were studied in two-choice bioassays. Gravid females laid a significantly higher percentage of eggs on substrates smeared with extract of Basilocal leaves (HEBL) (69%) than on those smeared with extracts of Kisan leaves (HEKL) (31%). Several chemicals were isolated from HEKL, three of which were characterized as dotriacontanol, heptadecanol, and nonadecanol. These chemicals were either absent or were present in very small amounts in HEBL, but in HEKL they were detected in much larger amounts. Each isolated chemical was tested for its effect on C. partellus oviposition in two-choice bioassays. Maximum ovipositional deterrence (90%) was observed for the compound MR-22a, followed in decreasing order by nonadecanol, MR-7, and heptadecanol. The identity of the remaining compounds is being investigated. The results indicate that the relative resistance of Kisan maize compared to Basilocal is partly due to the presence of certain ovipositional deterrents in its leaves.

摘要

在双选生物测定中，研究了亚洲玉米螟（Chilo partellus (Swinhoe)）（鳞翅目：螟蛾科）对两个玉米（Zea mays L.）品种叶片己烷提取物的产卵反应，其中一个是抗性品种（Kisan），另一个是感虫品种（Basilocal）。怀有身孕的雌蛾在涂抹了Basilocal叶片提取物（HEBL）的基质上产卵的比例（69%）显著高于涂抹了Kisan叶片提取物（HEKL）的基质（31%）。从HEKL中分离出了几种化学物质，其中三种被鉴定为三十二烷醇、十七烷醇和十九烷醇。这些化学物质在HEBL中要么不存在，要么含量极少，但在HEKL中检测到的含量要大得多。在双选生物测定中，对每种分离出的化学物质对亚洲玉米螟产卵的影响进行了测试。化合物MR - 22a观察到最大产卵抑制率（90%），其次按降序排列为十九烷醇、MR - 7和十七烷醇。其余化合物的身份正在研究中。结果表明，与Basilocal相比，Kisan玉米的相对抗性部分归因于其叶片中存在某些产卵抑制剂。