Weller R, Bateson M M, Heimbuch B K, Kopczynski E D, Ward D M
Department of Microbiology, Montana State University, Bozeman 59717.
Appl Environ Microbiol. 1992 Dec;58(12):3964-9. doi: 10.1128/aem.58.12.3964-3969.1992.
Analysis of 16S rRNA sequences retrieved as cDNA (16S rcDNA) from the Octopus Spring cyanobacterial mat has permitted phylogenetic characterization of some uncultivated community members, expanding our knowledge or diversity within this microbial community. Two new cyanobacterial 16S rRNA sequences were discovered, raising to four the number of cyanobacterial sequence types known to occur in the mat. None of the sequences found is that of the cultivated thermophilic cyanobacterium Synechococcus lividus. A new 16S rRNA sequence characteristic of green nonsulfur bacteria and their relatives was discovered, raising to two the number of such sequences known to exist in the mat. Both are unique among the 16S rRNA sequences of cultivated members of this group, including an Octopus Spring isolate of Chloroflexus aurantiacus and Heliothrix oregonensis, whose sequences we report herein. Two spirochete-like 16S rRNA sequences were discovered. One can be placed in the leptospira subdivision of the spirochete group, but the other has such a loose affiliation with the spirochete group that it might actually belong to an as yet unrecognized subdivision or even to a new eubacterial line of descent.
对从章鱼泉蓝细菌垫中作为cDNA检索到的16S rRNA序列（16S rcDNA）进行分析，使得一些未培养的群落成员得以进行系统发育特征分析，扩展了我们对这个微生物群落内多样性的认识。发现了两个新的蓝细菌16S rRNA序列，使已知在该菌垫中出现的蓝细菌序列类型数量增加到四个。所发现的序列均不是已培养的嗜热蓝细菌嗜热栖热放线菌（Synechococcus lividus）的序列。发现了一个新的绿色非硫细菌及其相关菌的16S rRNA序列特征，使已知存在于该菌垫中的此类序列数量增加到两个。这两个序列在该类群已培养成员的16S rRNA序列中都是独特的，包括我们在此报告其序列的章鱼泉橙黄嗜热栖热放线菌（Chloroflexus aurantiacus）分离株和俄勒冈嗜热栖热放线菌（Heliothrix oregonensis）。发现了两个类似螺旋体的16S rRNA序列。一个可以归入螺旋体群的钩端螺旋体亚群，但另一个与螺旋体群的关系非常松散，以至于它实际上可能属于一个尚未被认识的亚群，甚至属于一个新的确立的真细菌谱系。
