Ward D M, Weller R, Bateson M M
Department of Microbiology, Montana State University, Bozeman, MT.
FEMS Microbiol Rev. 1990 Jun;6(2-3):105-15. doi: 10.1111/j.1574-6968.1990.tb04088.x.
Molecular methods are beginning to reveal inhabitants of natural microbial communities which have never before been cultured. Our approach involves selective cloning of naturally occurring 16S rRNA sequences as cDNA, and comparison of these sequences to a database which includes 16S rRNA sequences of isolated community members. We provide here an overview of the method and its potential for community analysis. A 16S rRNA sequence retrieved from the well-studied hot spring cyanobacterial mat in Octopus Spring (Yellowstone National Park) is shown as an example of one contributed by an uncultured member of the community.
分子方法开始揭示天然微生物群落中以前从未培养过的成员。我们的方法包括将天然存在的16S rRNA序列选择性克隆为cDNA，并将这些序列与一个包含分离出的群落成员的16S rRNA序列的数据库进行比较。我们在此概述该方法及其在群落分析中的潜力。从章鱼泉（黄石国家公园）经过充分研究的温泉蓝藻垫中检索到的一个16S rRNA序列，作为群落中未培养成员贡献的一个例子展示出来。
