Koizumi H, Tanaka H, Fukaya T, Ohkawara A
Department of Dermatology, Hokkaido University School of Medicine, Sapporo, Japan.
Br J Dermatol. 1992 Dec;127(6):595-9. doi: 10.1111/j.1365-2133.1992.tb14872.x.
Substance P is a neuropeptide present in, and released from, peripheral C nerve endings. The presence of substance P-positive nerve fibres in the epidermis has been reported. We investigated the effect of substance P on the transmembrane signalling system of pig epidermal keratinocytes. Treatment of pig epidermis with substance P resulted in an increase in inositol 1,4,5-trisphosphate (IP3), and in intracellular free calcium. The treatment also resulted in translocation of protein kinase C from a cytosol to a membrane fraction. Substance P, however, did not affect the beta-adrenergic- or histamine (H2)- adenylate cyclase responses of the epidermis. Neither forskolin-induced, nor cholera toxin-induced cyclic AMP accumulation were affected by substance P treatment. These results consistent with the view that substance P stimulates phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis of keratinocytes, resulting in IP3-Ca2+ and diacylglycerol-protein kinase C signal activation. Although protein kinase C is known to affect the epidermal adenylate cyclase system, no evidence for such 'cross-talk regulation' was detected in keratinocytes by substance P treatment.
P物质是一种存在于外周C神经末梢并从其中释放的神经肽。已有报道表明表皮中存在P物质阳性神经纤维。我们研究了P物质对猪表皮角质形成细胞跨膜信号系统的影响。用P物质处理猪表皮会导致肌醇1,4,5-三磷酸（IP3）增加以及细胞内游离钙增加。该处理还导致蛋白激酶C从胞质溶胶转移至膜部分。然而，P物质并不影响表皮的β-肾上腺素能或组胺（H2）-腺苷酸环化酶反应。P物质处理既不影响福斯高林诱导的也不影响霍乱毒素诱导的环磷酸腺苷积累。这些结果与以下观点一致，即P物质刺激角质形成细胞的磷脂酰肌醇-4,5-二磷酸（PIP2）水解，导致IP3-Ca2+和二酰甘油-蛋白激酶C信号激活。尽管已知蛋白激酶C会影响表皮腺苷酸环化酶系统，但通过P物质处理在角质形成细胞中未检测到这种“相互作用调节”的证据。
