Hagstrom J, Harvey S, Wieben E
Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, Minnesota 55905.
Biol Reprod. 1992 Nov;47(5):768-75. doi: 10.1095/biolreprod47.5.768.
The guinea pig seminal vesicle epithelium (GPSVE) synthesizes and secretes milligram quantities of four related secretory proteins in an androgen-dependent manner. To investigate the role of androgens in the establishment of secretory protein synthesis during the development of the GPSVE, animals were castrated at Day 5, approximately 10 days before secretory protein accumulation begins in intact animals. Castration did not eliminate secretory protein mRNA from the SVE, but it did indefinitely postpone the developmentally programmed increase in secretory protein mRNA. Injection of neonatally castrated guinea pigs with either estradiol or dexamethasone did not alter levels of secretory protein mRNAs. However, treatment of castrated neonates with either testosterone propionate or dihydrotestosterone (DHT) led to specific increases in secretory protein mRNAs within 4 days. Although neonatally castrated animals accumulated and translated significant amounts of secretory protein mRNA, the newly synthesized secretory proteins failed to accumulate until exogenous androgens were provided. This observation suggests that androgens regulate both the accumulation of secretory protein mRNA and the accumulation of secretory proteins in the GPSVE.
豚鼠精囊上皮（GPSVE）以雄激素依赖的方式合成并分泌毫克量的四种相关分泌蛋白。为了研究雄激素在GPSVE发育过程中分泌蛋白合成建立过程中的作用，在第5天（即完整动物开始积累分泌蛋白前约10天）对动物进行去势。去势并未从精囊上皮中消除分泌蛋白mRNA，但确实无限期推迟了分泌蛋白mRNA在发育程序中的增加。给新生去势的豚鼠注射雌二醇或地塞米松均未改变分泌蛋白mRNA的水平。然而，用丙酸睾酮或二氢睾酮（DHT）处理去势的新生豚鼠，4天内分泌蛋白mRNA会有特异性增加。虽然新生去势动物积累并翻译了大量的分泌蛋白mRNA，但在提供外源性雄激素之前，新合成的分泌蛋白无法积累。这一观察结果表明，雄激素调节GPSVE中分泌蛋白mRNA的积累以及分泌蛋白的积累。
