Fawell S E, Higgins S J
Mol Cell Endocrinol. 1986 Nov;48(1):39-49. doi: 10.1016/0303-7207(86)90164-4.
The seminal vesicles of the rat synthesise large amounts of androgen-regulated secretory proteins. Indirect immunofluorescence cytochemistry and immunoblotting with monospecific polyclonal antibodies against three of the major secretory proteins (II, S and F) have been used to investigate the tissue distribution, subcellular localisation, androgen-regulation and developmental profile of secretory protein synthesis. There was no evidence for regional specialisation of the seminal vesicle epithelium; every epithelial cell synthesizes all three proteins via a classical secretory involving storage in secretory vesicles. Proteins S and II are contained within the same secretory vesicles. The time course of deinduction of proteins S and F after castration and their reinduction by testosterone closely followed that for their specific mRNAs described previously. During development, proteins S and F first appear between 10 and 15 days after birth. A protein immunologically related to seminal vesicle protein II is present in the lateral and dorsal lobes of the prostatic complex.
大鼠的精囊合成大量雄激素调节的分泌蛋白。已使用间接免疫荧光细胞化学和针对三种主要分泌蛋白（II、S和F）的单特异性多克隆抗体进行免疫印迹，以研究分泌蛋白合成的组织分布、亚细胞定位、雄激素调节和发育概况。没有证据表明精囊上皮存在区域特化；每个上皮细胞都通过经典的分泌途径合成这三种蛋白质，包括储存在分泌小泡中。蛋白质S和II包含在同一分泌小泡中。阉割后蛋白质S和F的去诱导时间进程以及它们被睾酮重新诱导的时间进程与先前描述的它们的特异性mRNA的时间进程密切相关。在发育过程中，蛋白质S和F在出生后10至15天之间首次出现。在前列腺复合体的侧叶和背叶中存在一种与精囊蛋白II免疫相关的蛋白质。
