木糖构型的寡核苷酸（XNA，木糖核酸）：构象受限衍生物的合成及其与DNA和RNA互补链的杂交
Xylo-Configured oligonucleotides (XNA, xylo nucleic acid): synthesis of conformationally restricted derivatives and hybridization towards DNA and RNA complements.
作者信息
Poopeiko Nicolai E, Juhl Martin, Vester Birte, Sørensen Mads D, Wengel Jesper
机构信息
Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, Campusvej 55, DK-5230, Odense M, Denmark.
出版信息
Bioorg Med Chem Lett. 2003 Jul 21;13(14):2285-90. doi: 10.1016/s0960-894x(03)00441-4.
Xylo-Configured oligonucleotides (XNA) containing a novel conformationally restricted 2'-deoxy-2'-fluoro-beta-D-xylofuranosyl nucleotide monomer, a novel conformationally locked 2'-amino-2'-deoxy-2'-N,4'-C-methylene-beta-D-xylofuranosyl nucleotide monomer, and a known 2'-deoxy-beta-D-xylofuranosyl nucleotide monomer (XNA monomers) have been synthesized and their hybridization towards DNA and RNA complements studied. Thermal denaturation studies of nine-mer mixed-base sequences composed of a mixture of XNA monomers and DNA monomers revealed preferential hybridization towards RNA complements relative to DNA complements. For 14-mer homo-thymine XNAs containing thirteen XNA monomers, stable complexes towards single-stranded DNA and RNA were formed at pH 7. Gel-shift experiments revealed these complexes to involve at least two XNA strands per DNA or RNA target strand.
已合成了包含新型构象受限的2'-脱氧-2'-氟-β-D-木糖呋喃糖核苷酸单体、新型构象锁定的2'-氨基-2'-脱氧-2'-N,4'-C-亚甲基-β-D-木糖呋喃糖核苷酸单体以及已知的2'-脱氧-β-D-木糖呋喃糖核苷酸单体（XNA单体）的木糖构型寡核苷酸（XNA），并研究了它们与DNA和RNA互补链的杂交情况。对由XNA单体和DNA单体混合物组成的九聚体混合碱基序列进行的热变性研究表明，相对于DNA互补链，其对RNA互补链具有优先杂交性。对于含有13个XNA单体的14聚体同胸腺嘧啶XNA，在pH 7时可与单链DNA和RNA形成稳定的复合物。凝胶迁移实验表明，这些复合物在每个DNA或RNA靶链中至少涉及两条XNA链。
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