Hof P R, Nimchinsky E A
Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, New York 10029.
Cereb Cortex. 1992 Nov-Dec;2(6):456-67. doi: 10.1093/cercor/2.6.456.
The cingulate cortex is composed of morphologically and functionally distinct areas. It is considered to be a major component of the limbic system and has been shown to subserve a wide range of autonomic and somatic motor functions. The anterior and posterior regions of the cingulate cortex can be differentiated according to their thalamic afferents as well as their patterns of corticocortical connectivity. The primate cingulate cortex is traditionally divided into a series of cytoarchitectonic zones that can be distinguished along a ventral-dorsal axis of differentiation in both the anterior (areas 25, 24a, 24b, and 24c), and posterior (areas 29, 30, 23a, 23b, and 23c) regions. However, little is known about the precise cellular organization of these subareas. In the present study, we attempt to define the neuronal morphological and biochemical composition of the different cingulate cortex subareas, using antibodies to the neurofilament triplet protein and calcium-binding proteins. Results indicate that there is a strong correlation between the structure and functions of the cingulate cortex and the immunostaining patterns. For instance, distribution of neurofilament-rich pyramidal neurons parallels that of specific corticocortical and corticosubcortical systems and is a useful marker to delineate the cingulate motor area. Calcium-binding protein-containing neurons display a high degree of regional and laminar specialization. In particular, parvalbumin-positive interneurons are codistributed with neurofilament-immunoreactive pyramidal cells along the ventrodorsal and rostrocaudal axes of the cingulate cortex. Calbindin- and calretinin-positive immunostaining show more monotonous laminar and regional patterns, although they exhibit a particular labeling in area 29 that may correspond to the termination of select thalamocortical afferents. These chemoarchitectural patterns of regional and laminar neuronal specialization may be envisioned as the reflection of the richness of cortical diversity in the cingulate gyrus, and make it an ideal place to explore the interplay of the distributions of various neuron types in cortical areas of known function.
扣带回皮质由形态和功能上不同的区域组成。它被认为是边缘系统的主要组成部分，并且已被证明能支持广泛的自主和躯体运动功能。扣带回皮质的前部和后部区域可根据它们的丘脑传入以及皮质-皮质连接模式进行区分。传统上，灵长类动物的扣带回皮质被分为一系列细胞构筑区，这些区域在前部（25区、24a区、24b区和24c区）和后部（29区、30区、23a区、23b区和23c区）沿腹-背分化轴均可区分。然而，对于这些亚区精确的细胞组织了解甚少。在本研究中，我们试图使用针对神经丝三联体蛋白和钙结合蛋白的抗体来确定不同扣带回皮质亚区的神经元形态和生化组成。结果表明，扣带回皮质的结构和功能与免疫染色模式之间存在很强的相关性。例如，富含神经丝的锥体神经元的分布与特定的皮质-皮质和皮质-皮质下系统的分布平行，并且是描绘扣带回运动区的有用标记。含钙结合蛋白的神经元表现出高度的区域和层状特异性。特别是，小白蛋白阳性中间神经元与神经丝免疫反应性锥体细胞沿扣带回皮质的腹-背和头-尾轴共同分布。钙结合蛋白和钙视网膜蛋白阳性免疫染色显示出更单调的层状和区域模式，尽管它们在29区表现出特定的标记，这可能对应于特定丘脑皮质传入纤维的终止。区域和层状神经元特异性的这些化学构筑模式可被视为扣带回中皮质多样性丰富性的反映，并使其成为探索已知功能皮质区域中各种神经元类型分布相互作用的理想场所。
