在蘑菇酪氨酸酶检测系统中熊果苷氧化产物的鉴定。
Identification of oxidation product of arbutin in mushroom tyrosinase assay system.
作者信息
Nihei Ken ichi, Kubo Isao
机构信息
Department of Environmental Science, Policy and Management, University of California, CA 94720-3112, Berkeley, USA.
出版信息
Bioorg Med Chem Lett. 2003 Jul 21;13(14):2409-12. doi: 10.1016/s0960-894x(03)00395-0.
In order to elucidate whitening mechanisms of arbutin (hydroquinone-O-beta-D-glucopyranoside), its effects on mushroom tyrosinase were analyzed by spectrophotometric, polarographic, and HPLC experiments. It was found that as soon as catalytic amounts of L-DOPA become available as a cofactor, arbutin acts as a monophenol substrate. A significant enzymatic product was identified as 3,4-dihydroxyphenyl-O-beta-D-glucopyranoside by NMR and MS experiments.
为阐明熊果苷（对苯二酚 - O - β - D - 吡喃葡萄糖苷）的美白机制，通过分光光度法、极谱法和高效液相色谱实验分析了其对蘑菇酪氨酸酶的影响。结果发现，一旦有催化量的L - 多巴作为辅因子可用，熊果苷就作为单酚底物起作用。通过核磁共振和质谱实验确定一种重要的酶促产物为3,4 - 二羟基苯基 - O - β - D - 吡喃葡萄糖苷。
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