Stark Alexander, Russell Robert B
EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Nucleic Acids Res. 2003 Jul 1;31(13):3341-4. doi: 10.1093/nar/gkg506.
The detection of local structural patterns in proteins (e.g. active sites) can provide insights into protein function in the absence of sequence or fold similarity. Methods to detect such similarities are key during structural annotation, for example with results from Structural Genomics initiatives. PINTS (Patterns in Non-homologous Tertiary Structures, http://pints.embl.de) performs database searches for such patterns and most importantly provides a measure of statistical significance for any similarity uncovered. To aid functional annotation of proteins, we allow comparisons of pre-defined patterns against databases of complete structures and of entire structures to databases of particular residues likely to be functionally important.
在缺乏序列或折叠相似性的情况下，检测蛋白质中的局部结构模式（如活性位点）有助于深入了解蛋白质功能。检测此类相似性的方法在结构注释过程中至关重要，例如对于结构基因组学计划的结果而言。PINTS（非同源三级结构中的模式，http://pints.embl.de）可针对此类模式进行数据库搜索，最重要的是，对于所发现的任何相似性，它都能提供统计显著性的度量。为了辅助蛋白质的功能注释，我们允许将预定义模式与完整结构数据库进行比较，并将整个结构与可能具有重要功能的特定残基数据库进行比较。
