神经肽Y对高饮酒量（HAD）和低饮酒量（LAD）大鼠蔗糖和乙醇摄入量以及焦虑样行为的影响。
Effects of neuropeptide Y on sucrose and ethanol intake and on anxiety-like behavior in high alcohol drinking (HAD) and low alcohol drinking (LAD) rats.
作者信息
Badia-Elder N E, Stewart R B, Powrozek Teresa A, Murphy J M, Li T-K
机构信息
Department of Psychology, Purdue School of Science, IUPUI, Indianapolis, Indiana, 46202 USA.
出版信息
Alcohol Clin Exp Res. 2003 Jun;27(6):894-9. doi: 10.1097/01.ALC.0000071929.17974.DA.
BACKGROUND
In a previous study, neuropeptide Y (NPY) administered into the lateral ventricles decreased ethanol intake in alcohol-preferring (P) rats but not in alcohol-nonpreferring (NP) or unselected Wistar rats. The purpose of the present investigation is to extend these findings in selectively-bred high-alcohol-drinking (HAD)1 and low-alcohol-drinking (LAD)1 rats by examining the effects of intracerebroventricularly administered NPY on the elevated plus maze test of anxiety and on ethanol and sucrose intake.
METHODS
Female HAD1 and LAD1 rats were surgically implanted with cannula into the lateral ventricle. Following recovery, a test of anxiety was conducted in which the rats (n = 12-13/group) received either artificial cerebrospinal fluid (aCSF) or NPY (10 microg) 10 min prior to a 5-min test on an elevated plus maze. Following anxiety testing, 11 HAD and 11 LAD rats were trained to self-administer ethanol (8% w/v), and 5 HAD and 8 LAD rats were trained to self-administer sucrose (2.5%) during daily 2-hr sessions. A within-subject design was used in which the rats were pretreated once a week with aCSF, 5 microg NPY, or 10 microg NPY prior to the drinking sessions.
RESULTS
HAD and LAD rats treated with aCSF did not differ in time spent in open arms of the plus maze. NPY increased time spent on the open arms to similar degrees in both rat lines. HAD rats consumed more ethanol and sucrose than LAD rats. NPY increased sucrose intake in both rat lines. However, the same doses of NPY reduced ethanol intake in HAD but not in LAD rats.
CONCLUSION
The plus maze results indicated that selective breeding for high and low alcohol preference in the HAD1 and LAD1 rats, respectively, did not yield differences in anxiety-like behavior and in response to the anxiolytic effects of NPY. The increases in sucrose intake were consistent with the known orexigenic effects of NPY. The decreased ethanol intake following NPY administration in HAD rats was similar to previous observations with P rats and is consistent with the hypothesis that ethanol intake and NPY activity may be inversely related.
背景
在之前的一项研究中，向侧脑室注射神经肽Y（NPY）可减少嗜酒（P）大鼠的乙醇摄入量，但对非嗜酒（NP）大鼠或未经过选择的Wistar大鼠没有影响。本研究的目的是通过检测脑室内注射NPY对高架十字迷宫焦虑试验以及乙醇和蔗糖摄入量的影响，在选择性培育的高饮酒量（HAD）1和低饮酒量（LAD）1大鼠中扩展这些发现。
方法
对雌性HAD1和LAD1大鼠进行手术，将套管植入侧脑室。恢复后，进行焦虑试验，在高架十字迷宫上进行5分钟测试前10分钟，给大鼠（每组n = 12 - 13）注射人工脑脊液（aCSF）或NPY（10微克）。焦虑测试后，11只HAD大鼠和11只LAD大鼠接受训练以自我注射乙醇（8% w/v），5只HAD大鼠和8只LAD大鼠接受训练以在每天2小时的时段内自我注射蔗糖（2.5%）。采用受试者内设计，在饮酒时段前，大鼠每周接受一次aCSF、5微克NPY或10微克NPY预处理。
结果
接受aCSF处理的HAD和LAD大鼠在高架十字迷宫开放臂上花费的时间没有差异。NPY使两个品系大鼠在开放臂上花费的时间增加到相似程度。HAD大鼠比LAD大鼠消耗更多的乙醇和蔗糖。NPY增加了两个品系大鼠的蔗糖摄入量。然而，相同剂量的NPY减少了HAD大鼠的乙醇摄入量，但对LAD大鼠没有影响。
结论
高架十字迷宫试验结果表明，分别对HAD1和LAD1大鼠进行高酒精偏好和低酒精偏好的选择性培育，在焦虑样行为以及对NPY抗焦虑作用的反应方面没有差异。蔗糖摄入量的增加与NPY已知的促食欲作用一致。HAD大鼠注射NPY后乙醇摄入量的减少与之前对P大鼠的观察结果相似，并且与乙醇摄入量和NPY活性可能呈负相关的假设一致。
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