Dimitriadis V K, Domouhtsidou G P, Raftopoulou E
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Greece.
Environ Pollut. 2003;125(3):345-53. doi: 10.1016/s0269-7491(03)00122-2.
The intracellular localization of heavy metals using autometallography (AMG) and X-ray microanalysis was studied in the palps, the digestive gland and the gills of mussels Mytilus galloprovincialis, after an experimental exposure to 0.1 mg l(-1) of Hg and 0.1 mg l(-1) of Pb, for 30 and 60 days. In the examined tissues, autometallographical black silver deposits (BSD) were localized mainly in the residual bodies and heterolysosomes of the digestive cells, as well as in the dense bodies of the epithelial cells. Metal deposition after Hg exposure was much more abundant compared to Pb exposure. Using X-ray microanalysis, Hg was traced on the BSD in all examined tissues, while Pb was not traced in these deposits. The results are discussed in comparison to previous results on long-term exposure to the same metals; in addition, the palps are introduced as a new metal storing organ and, finally, the use of X-ray microanalysis under a scanning electron microscope in order to enhance the specificity of AMG is suggested.
在将实验性暴露于0.1 mg l⁻¹的汞和0.1 mg l⁻¹的铅中30天和60天后，利用自动金相显微镜（AMG）和X射线微分析研究了加利福尼亚贻贝（Mytilus galloprovincialis）的触须、消化腺和鳃中重金属的细胞内定位。在所检查的组织中，自动金相显微镜下的黑色银沉积物（BSD）主要定位于消化细胞的残余体和异溶酶体中，以及上皮细胞的致密体中。与铅暴露相比，汞暴露后的金属沉积要丰富得多。利用X射线微分析，在所有检查的组织中，在BSD上都检测到了汞，而在这些沉积物中未检测到铅。将这些结果与先前关于长期暴露于相同金属的结果进行了比较讨论；此外，触须被作为一种新的金属储存器官引入，最后，建议在扫描电子显微镜下使用X射线微分析以提高AMG的特异性。
