Bramucci M, Kane H, Chen M, Nagarajan V
Central Research and Development, DuPont Company, P.O. Box 80328, Wilmington, DE 19880-0328, USA.
Appl Microbiol Biotechnol. 2003 Oct;62(5-6):594-600. doi: 10.1007/s00253-003-1372-x. Epub 2003 Jun 24.
Industrial wastewater bioreactors are potentially important sources of novel biocatalysts. However, the microbial populations in these bioreactors are not well characterized. The microbial community in an industrial wastewater bioreactor was surveyed by extracting DNA from a sample of activated sludge, followed by PCR amplification and sequencing of cloned 16S rRNA genes. A total of 407 cloned 16S rRNA gene sequences were compared with 88 bacterial isolates cultured from the same sample of sludge using a variety of standard media. Most of the bacteria detected by the PCR-based approach were beta-subdivision Proteobacteria, whereas most of the cultured bacteria were gamma-subdivision Proteobacteria. Only a few types of bacteria were detected by both approaches. These observations indicate that multiple techniques are necessary to characterize the microbial diversity in any complex ecosystem.
工业废水生物反应器有可能是新型生物催化剂的重要来源。然而，这些生物反应器中的微生物群落尚未得到充分表征。通过从活性污泥样本中提取DNA，随后对克隆的16S rRNA基因进行PCR扩增和测序，对工业废水生物反应器中的微生物群落进行了调查。使用各种标准培养基，将总共407个克隆的16S rRNA基因序列与从同一样本污泥中培养的88株细菌分离株进行了比较。基于PCR的方法检测到的大多数细菌是β-变形菌纲，而培养的大多数细菌是γ-变形菌纲。两种方法仅检测到少数几种细菌类型。这些观察结果表明，需要多种技术来表征任何复杂生态系统中的微生物多样性。
