Division of Microbial Resources, Campinas University - UNICAMP CP 6171, Campinas, SP, Brazil.
J Microbiol Biotechnol. 2010 Jan;20(1):21-9.
The phenolic compounds are a major contaminant class often found in industrial wastewaters and the biological treatment is an alternative tool commonly employed for their removal. In this sense, monitoring microbial community dynamics is crucial for a successful wastewater treatment. This work aimed to monitor the structure and activity of the bacterial community during the operation of a laboratory-scale continuous submerged membrane bioreactor (SMBR), using PCR and RT-PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA libraries. Multivariate analyses carried out using DGGE profiles showed significant changes in the total and metabolically active dominant community members during the 4-week treatment period, explained mainly by phenol and ammonium input. Gene libraries were assembled using 16S rDNA and 16S rRNA PCR products from the fourth week of treatment. Sequencing and phylogenetic analyses of clones from 16S rDNA library revealed a high diversity of taxa for the total bacterial community, with predominance of Thauera genus (ca. 50%). On the other hand, a lower diversity was found for metabolically active bacteria, which were mostly represented by members of Betaproteobacteria (Thauera and Comamonas), suggesting that these groups have a relevant role in the phenol degradation during the final phase of the SMBR operation.
酚类化合物是工业废水中常见的主要污染物类别之一，生物处理是去除它们的常用替代工具。在这种情况下，监测微生物群落动态对于成功处理废水至关重要。本研究旨在使用聚合酶链式反应（PCR）和反转录聚合酶链式反应（RT-PCR）以及变性梯度凝胶电泳（DGGE）和 16S rRNA 文库，监测实验室规模连续浸没式膜生物反应器（SMBR）运行过程中细菌群落的结构和活性。使用 DGGE 图谱进行的多元分析表明，在 4 周的处理期间，总群落和代谢活跃的优势群落成员发生了显著变化，主要与苯酚和氨输入有关。使用第 4 周处理的 16S rDNA 和 16S rRNA PCR 产物组装基因文库。从 16S rDNA 文库克隆的测序和系统发育分析显示，总细菌群落的分类多样性很高，Thauera 属占优势（约 50%）。另一方面，代谢活跃细菌的多样性较低，主要由 Betaproteobacteria（Thauera 和 Comamonas）的成员代表，表明这些菌群在 SMBR 运行的最后阶段对苯酚降解具有重要作用。
