Braun Ruedi K, Foerster Martin, Grahmann P Reinhard, Haefner Daniela, Workalemahu Grefachew, Kroegel Claus
Ottawa Health Research Institute, Ottawa, Ontario, Canada.
Cytometry B Clin Cytom. 2003 Jul;54(1):19-27. doi: 10.1002/cyto.b.10021.
The integrin CD103 is preferentially expressed on intraepithelial T lymphocytes, and cells expressing this integrin may play a regulatory role in the microenvironment of the epithelial cell layer.
The relative number of CD103(+)/CD4(+) T cells in the bronchoalveolar lavage was significantly elevated in all patients diagnosed with interstitial lung diseases compared with patients with other non-fibrotic disorders of the lung.
Analysis by flow cytometry showed that the CD103(+) and the CD103(-) subpopulations were memory T cells based on the high expression of CD45RO(+). However, the CD103(+)/CD4(+) T cells were CD25(low), CD27(-), CD28(low), and CD62L(-), whereas the CD103(-)/CD4(+) T cells expressed CD25 and CD62L and were CD27(high) and CD28(high). In addition, the CD103(+)/CD4(+) T cells expressed significantly higher quantities of VLA-1 and CD101 than did CD103(-)/CD4(+) T cells. Reverse transcriptase polymerase chain reaction analysis of purified CD103(+) and CD103(-) CD4(+) T cells showed production of tumor necrosis factor (TNF) alpha-R-1 (p55), TNF-alpha-R-2 (p75), interferon gamma, interleukin-10, and TNF-alpha mRNA in both subpopulations. No interleukin-4 mRNA was detected in either subpopulation.
CD103(+)/CD4(+) T cells represent a T-helper 1-like subpopulation in human lungs with a distinct effector phenotype. Despite the lack of CD27 and the low CD25 and CD28 expression, these cells show a high degree of activation. These results suggest that CD103 expressing CD4 T cells in the lung are continuously activated, long-living cells.
整合素CD103优先表达于上皮内T淋巴细胞，表达该整合素的细胞可能在上皮细胞层的微环境中发挥调节作用。
与其他非纤维化肺部疾病患者相比，所有诊断为间质性肺疾病的患者支气管肺泡灌洗中CD103(+)/CD4(+) T细胞的相对数量显著升高。
流式细胞术分析显示，基于CD45RO(+)的高表达，CD103(+)和CD103(-)亚群均为记忆T细胞。然而，CD103(+)/CD4(+) T细胞为CD25(低)、CD27(-)、CD28(低)和CD62L(-)，而CD103(-)/CD4(+) T细胞表达CD25和CD62L，且为CD27(高)和CD28(高)。此外，CD103(+)/CD4(+) T细胞表达的VLA-1和CD101量显著高于CD103(-)/CD4(+) T细胞。对纯化的CD103(+)和CD103(-) CD4(+) T细胞进行逆转录聚合酶链反应分析显示，两个亚群均产生肿瘤坏死因子(TNF)α-R-1(p55)、TNF-α-R-2(p75)、干扰素γ、白细胞介素-10和TNF-α mRNA。两个亚群均未检测到白细胞介素-4 mRNA。
CD103(+)/CD4(+) T细胞代表人类肺部具有独特效应表型的类似辅助性T细胞1亚群。尽管缺乏CD27且CD25和CD28表达较低，但这些细胞显示出高度活化。这些结果表明，肺中表达CD103的CD4 T细胞是持续活化的长寿细胞。
