Pasqualin A, Tsukahara T, Hongo K, Van Beek O, Kassell N F, Torner J C
Department of Neurological Surgery, University of Virginia Health Sciences Center, Charlottesville.
Acta Neurochir (Wien). 1992;119(1-4):139-45. doi: 10.1007/BF01541798.
The vasoactive effects of substance P (SP), as well as the content of cyclic guanine monophosphate (cGMP), were determined in the rabbit basilar artery after subarachnoid haemorrhage (SAH). Out of 47 rabbits, 24 were subjected to a SAH, induced by injecting 5 ml of autologous arterial blood into the cisterna magna; 23 were used as controls. In 20 animals (10 SAH and 10 controls), isometric tension recording of isolated rings of the basilar artery--dissected 2 days after SAH--was employed to assess the dose-dependent vasodilatation to SP (10(-10) to 10(-6) M) after precontraction with serotonin (10(-8) to 10(-5) M). In 15 animals (8 SAH and 7 controls), the basal cGMP content was measured in the basilar artery 2 days after SAH. In the other 12 animals (6 SAH and 6 controls), the increase in cGMP content was measured in the basilar artery after a 10-minute incubation with SP (10(-6) M). SP caused significantly less dilatation in animals subjected to SAH than in controls, especially for concentrations between 10(-9) and 10(-6) M (p < 0.001). The cGMP content in the arteries 2 days after SAH was significantly lower than in control arteries (31.5 +/- 7.3 against 57.3 +/- 4.3 pmoles/g tissue). In the preparations incubated with SP, the increase of cGMP was 440 +/- 115% in the control arteries, and only 97 +/- 30% in the arteries after SAH. It is concluded that the vasodilator activity of SP is significantly impaired after SAH.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了蛛网膜下腔出血（SAH）后兔基底动脉中P物质（SP）的血管活性作用以及环磷酸鸟苷（cGMP）的含量。47只兔中，24只通过向小脑延髓池注入5ml自体动脉血诱导SAH；23只作为对照。在20只动物（10只SAH和10只对照）中，采用等长张力记录法，对SAH后2天分离的基底动脉环进行研究，以评估在预先用5-羟色胺（10⁻⁸至10⁻⁵M）预收缩后，对SP（10⁻¹⁰至10⁻⁶M）的剂量依赖性血管舒张作用。在15只动物（8只SAH和7只对照）中，测量SAH后2天基底动脉中的基础cGMP含量。在另外12只动物（6只SAH和6只对照）中，测量基底动脉在与SP（10⁻⁶M）孵育10分钟后的cGMP含量增加情况。与对照相比，SAH动物中SP引起的舒张作用明显减弱，尤其是在10⁻⁹至10⁻⁶M浓度之间（p<0.001）。SAH后2天动脉中的cGMP含量明显低于对照动脉（分别为31.5±7.3和57.3±4.3pmol/g组织）。在用SP孵育的制剂中，对照动脉中cGMP的增加为440±115%，而SAH后动脉中仅为97±30%。得出结论：SAH后SP的血管舒张活性明显受损。（摘要截短于250字）
