Komori M, Kikuchi O, Sakuma T, Funaki J, Kitada M, Kamataki T
Division of Analytical Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.
Biochim Biophys Acta. 1992 Dec 29;1171(2):141-6. doi: 10.1016/0167-4781(92)90113-e.
Three cDNAs coding for monkey cytochrome P-450 (P450) 2C, 2E and 3A (MKmp13, MKj1 and MKnf2, respectively) were isolated from a lambda gt11 cDNA library of a liver from a 3-methylcholanthrene (3MC)-treated crab-eating monkey, using cDNA fragments for human P450 2C, 2E and 3A as respective probes. MKmp13 and MKnf2 were 1901 and 2032 bp long, containing entire coding regions for polypeptides of 490 and 503 residues, respectively. The deduced N-terminal amino acid sequences of MKmp13 and MKnf2 were identical with those of P450-MK1 and P450-MK2, which had been purified from liver microsomes of untreated and polychlorinated biphenyl (PCB)-treated crab-eating monkeys, respectively. MKj1 was 1508 bp long, encoding a polypeptide of 449 residues, which is presumed to lack N-terminal 45 residues as compared with the sequence for human P450 2E1. Northern blot analysis indicated that monkey P450 2C, 2E and 3A mRNAs were expressed constitutively in monkey livers. P450 2E and 3A mRNAs were induced by both 3MC and PCB, while P450 2C mRNA was induced only by PCB. The deduced amino acid sequences of four monkey cytochrome P-450 cDNAs, including P450 1A1 (MKah1) which we isolated previously, were more than 92% identical with those of corresponding human cytochrome P-450 cDNAs.
从一只经3-甲基胆蒽（3MC）处理的食蟹猴肝脏的λgt11 cDNA文库中，分别使用人细胞色素P-450（P450）2C、2E和3A的cDNA片段作为探针，分离出了编码猴细胞色素P-450 2C、2E和3A（分别为MKmp13、MKj1和MKnf2）的三个cDNA。MKmp13和MKnf2长度分别为1901和2032 bp，分别包含490和503个残基的多肽的完整编码区。MKmp13和MKnf2推导的N端氨基酸序列分别与P450-MK1和P450-MK2的相同，P450-MK1和P450-MK2分别从未经处理和经多氯联苯（PCB）处理的食蟹猴肝脏微粒体中纯化得到。MKj1长度为1508 bp，编码一个449个残基的多肽，与人类P450 2E1的序列相比，推测其缺少N端45个残基。Northern印迹分析表明，猴P450 2C、2E和3A mRNA在猴肝脏中组成性表达。P450 2E和3A mRNA可被3MC和PCB诱导，而P450 2C mRNA仅被PCB诱导。包括我们之前分离的P450 1A1（MKah1）在内的四个猴细胞色素P-450 cDNA推导的氨基酸序列与相应的人细胞色素P-450 cDNA的序列一致性超过92%。
