Stasíková J, Kutinová L, Smahel M, Nĕmecková S
Department of Experimental Virology, Institute of Hematology and Blood Transfusion, U nemocnice 1, 128 20 Prague 2, Czech Republic.
Acta Virol. 2003;47(1):1-10.
Immunization with DNA vaccines expressing Varicella-zoster virus (VZV) glycoprotein E (gE) induced formation of specific antibodies in mice. The antibody response correlated with the level of in vitro gE expression if the plasmid was inoculated intradermally (i.d.) with a gene gun but not if intramuscular (i.m.) injection was used. The i.d. vaccination produced a higher antibody level than the i.m. one even though a 100-fold amount of DNA was administered. A plasmid expressing a truncated form of gE was less immunogenic. The magnitude of antibody response induced by immunization with recombinant vaccinia viruses (rVVs) was equivalent to the gene gun vaccination. Administration of DNA by i.m. route or Vaccinia virus (VV) gE by i.d. mute resulted in predominance of IgG2a in the response while the gene gun plasmid inoculation usually elicited similar levels of IgG1 and IgG2a. The antibody response elicited by DNA vaccine was boosted by a secondary immunization with rVV. The boosting effect was highest if the virus was administered intraperitoneal (i.p.).
用表达水痘 - 带状疱疹病毒（VZV）糖蛋白E（gE）的DNA疫苗免疫可在小鼠体内诱导特异性抗体的形成。如果用基因枪皮内（i.d.）接种质粒，抗体反应与体外gE表达水平相关，但如果采用肌肉内（i.m.）注射则不相关。即使给予的DNA量增加100倍，皮内接种产生的抗体水平仍高于肌肉内接种。表达截短形式gE的质粒免疫原性较低。用重组痘苗病毒（rVVs）免疫诱导的抗体反应强度与基因枪接种相当。通过肌肉内途径给予DNA或通过皮内途径给予痘苗病毒（VV）gE会导致反应中IgG2a占主导，而基因枪接种质粒通常会引发相似水平的IgG1和IgG2a。DNA疫苗引发的抗体反应通过用rVV进行二次免疫得到增强。如果通过腹腔内（i.p.）给予病毒，增强效果最高。
