Ludvíková V, Kunke D, Hamsíková E, Kutinová L, Vonka V
Department of Experimental Virology, Institute of Sera and Vaccines, Prague, Czechoslovakia.
J Gen Virol. 1991 Jun;72 ( Pt 6):1445-9. doi: 10.1099/0022-1317-72-6-1445.
Several vaccinia virus (VV)-varicella-zoster virus (VZV) recombinants expressing glycoprotein I (gpI) of VZV were isolated from the Prague strain of VV. One of these, v46, was inoculated intraperitoneally into mice. Groups of mice were bled 4 and 8 weeks later and their sera were examined for anti-VZV and anti-VV antibodies by ELISA. At 4 weeks, all mice inoculated with the three largest virus doses (10(7), 10(6) and 10(5) p.f.u.), and at 8 weeks all mice inoculated with the four highest virus doses (10(7), 10(6), 10(5), and 10(4) p.f.u.), had developed both anti-VV and anti-VZV antibodies. Antibodies were also detected in a high proportion of mice infected with lower doses of virus and in some instances VZV antibodies were present in the absence of VV antibodies. None of the animals inoculated in parallel with either a thymidine kinase-negative mutant of the original VV or diluent alone developed antibody reactive with VZV. The specificity of the reaction was assessed further by Western blotting using anti-gpI monoclonal antibodies as a positive control. Sera from animals immunized with v46 possessed antibody capable of neutralizing extracellular VZV in the presence of complement.
从痘苗病毒（VV）的布拉格株中分离出几种表达水痘带状疱疹病毒（VZV）糖蛋白I（gpI）的VV-VZV重组体。其中之一v46经腹腔接种到小鼠体内。在4周和8周后对几组小鼠进行采血，并通过酶联免疫吸附测定（ELISA）检测其血清中的抗VZV和抗VV抗体。在4周时，接种了三种最大病毒剂量（10⁷、10⁶和10⁵蚀斑形成单位）的所有小鼠，以及在8周时接种了四种最高病毒剂量（10⁷、10⁶、10⁵和10⁴蚀斑形成单位）的所有小鼠，均产生了抗VV和抗VZV抗体。在感染较低剂量病毒的高比例小鼠中也检测到了抗体，并且在某些情况下，在没有VV抗体的情况下也存在VZV抗体。与原始VV的胸苷激酶阴性突变体或单独的稀释剂平行接种的动物均未产生与VZV反应的抗体。以抗gpI单克隆抗体作为阳性对照，通过蛋白质印迹法进一步评估反应的特异性。用v46免疫的动物血清中所含的抗体在有补体存在的情况下能够中和细胞外VZV。
