牙龈卟啉单胞菌感染的牙周炎和动脉粥样硬化患者对热休克蛋白的免疫反应。
Immune responses to heat shock protein in Porphyromonas gingivalis-infected periodontitis and atherosclerosis patients.
作者信息
Chung Sung-Woon, Kang Ho-Sung, Park Hae-Ryoun, Kim Sung-Jo, Kim Soo-Jin, Choi Jeom-Il
机构信息
Department of Thoracic and Cardiovascular Surgery, School of Medicine, College of Natural Sciences, Pusan, Korea.
出版信息
J Periodontal Res. 2003 Aug;38(4):388-93. doi: 10.1034/j.1600-0765.2003.00664.x.
BACKGROUND
It has been widely thought that heat shock protein might be involved in autoimmune disease mechanisms in humans.
OBJECTIVES
The present study was performed to evaluate the recognition of Porphyromonas gingivalis heat shock protein 60 (hsp60) and human hsp60 by immune sera in P. gingivalis-infected periodontitis and atherosclerosis patients.
MATERIALS AND METHODS
Mononuclear cells from atheroma lesions were stimulated with P. gingivalis hsp and sera from periodontitis or atherosclerosis patients were subjected to Western immunoblotting to P. gingivalis hsp or human hsp, respectively.
RESULTS
Western immunoblot analysis demonstrated the dual reactivity of anti-P. gingivalis antisera with P. gingivalis hsp and human hsp. We could also establish P. gingivalis hsp-specific T cell lines from the atheroma lesions, a mixture of CD4+ and CD8+ cells producing the cytokines characteristic of both Th1 and Th2 subsets.
CONCLUSION
These observations suggest the modulating effect of P. gingivalis hsp60 in the immunopathogenesis of periodontitis and atherosclerosis.
背景
人们普遍认为热休克蛋白可能参与人类自身免疫性疾病机制。
目的
本研究旨在评估牙龈卟啉单胞菌热休克蛋白60（hsp60）和人hsp60在牙龈卟啉单胞菌感染的牙周炎和动脉粥样硬化患者中被免疫血清识别的情况。
材料与方法
用牙龈卟啉单胞菌热休克蛋白刺激动脉粥样硬化病变中的单核细胞，分别用牙周炎或动脉粥样硬化患者的血清对牙龈卟啉单胞菌热休克蛋白或人热休克蛋白进行Western免疫印迹分析。
结果
Western免疫印迹分析表明，抗牙龈卟啉单胞菌抗血清对牙龈卟啉单胞菌热休克蛋白和人热休克蛋白具有双重反应性。我们还可以从动脉粥样硬化病变中建立牙龈卟啉单胞菌热休克蛋白特异性T细胞系，这是一种CD4+和CD8+细胞的混合物，可产生Th1和Th2亚群特征性的细胞因子。
结论
这些观察结果提示牙龈卟啉单胞菌hsp60在牙周炎和动脉粥样硬化的免疫发病机制中具有调节作用。
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