Singh Pratik, Tripathy Deoki N
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Viral Immunol. 2003;16(2):223-7. doi: 10.1089/088282403322017956.
While attenuated fowlpox virus (FPV) strains are widely used for vaccination of chickens and turkeys for prevention of fowlpox, recombinant FPV expressing various foreign genes have been evaluated for their ability to offer protection against various diseases in poultry as well as mammals. Little is known regarding the cell-mediated immune responses to FPV infection. In this study, immune response in chickens infected with a virulent and a vaccine strain of FPV were compared by a lymphoproliferation assay. Interestingly, a lymphoproliferative response was seen during 2-4 weeks post-infection irrespective of the FPV strain used in this study. Analyses of the buffy coat cultures with (35)S-methionine pulse labeling revealed an elevated protein of approximately 48-50 kDa in the culture supernatants. Furthermore, those supernatants could stimulate naive, non-adherent cells of the buffy coat cultures, in a dose dependant manner, suggestive of stimulatory cytokines. FPV, a complex virus presumably stimulates a variety of cytokines in vivo causing a proliferative cellular response. Knowledge of those cytokines or a better understanding of the proliferative responses is pivotal in evaluation of FPV vaccines and in the design of FPV-based recombinant vaccines.
虽然减毒鸡痘病毒（FPV）株广泛用于鸡和火鸡的疫苗接种以预防鸡痘，但表达各种外源基因的重组FPV已被评估其在预防家禽以及哺乳动物的各种疾病方面提供保护的能力。关于对FPV感染的细胞介导免疫反应知之甚少。在本研究中，通过淋巴细胞增殖试验比较了感染强毒和疫苗株FPV的鸡的免疫反应。有趣的是，无论本研究中使用的FPV株如何，在感染后2至4周期间均观察到淋巴细胞增殖反应。用（35）S-甲硫氨酸脉冲标记对血沉棕黄层培养物进行分析，结果显示培养上清液中一种约48-50 kDa的蛋白质含量升高。此外，这些上清液可以剂量依赖性方式刺激血沉棕黄层培养物中的幼稚非贴壁细胞，提示有刺激细胞因子存在。FPV是一种复杂病毒，可能在体内刺激多种细胞因子，引起细胞增殖反应。了解这些细胞因子或更好地理解增殖反应对于评估FPV疫苗和设计基于FPV的重组疫苗至关重要。
