Nagy E, Maeda-Machang'u A D, Krell P J, Derbyshire J B
Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
Avian Dis. 1990 Jul-Sep;34(3):677-82.
Administration of 10(4) mean cell-culture infectious dose (CCID50) per ml of a plaque-purified derivative of a commercial fowlpox virus (FPV) vaccine to 1-day-old chicks by aerosol or drinking water gave inconsistent serological responses and little evidence of protective immunity. In contrast, cutaneous vaccination with the same preparation protected against challenge with virulent FPV at 4 weeks of age. Administration of the vaccine at a concentration of 10(6) CCID50 per ml by the drinking-water route was as effective as conventional cutaneous vaccination in terms of the serological response in an enzyme-linked immuno-sorbent assay and in terms of protection against challenge. Drinking-water vaccination at 2 days of age was no more effective than vaccination on day 1, and oral dosing with the vaccine was less effective than incorporation of the vaccine in the drinking water. It was concluded that 1-day-old chicks may be vaccinated against fowlpox by the drinking-water route if the vaccine contains a sufficiently high concentration of virus.
给1日龄雏鸡通过气溶胶或饮水途径接种每毫升含10(4)个平均细胞培养感染剂量（CCID50）的商业鸡痘病毒（FPV）疫苗空斑纯化衍生物，其血清学反应不一致，几乎没有保护性免疫的证据。相比之下，用相同制剂进行皮肤接种可在4周龄时抵御强毒FPV的攻击。就酶联免疫吸附测定中的血清学反应以及抵御攻击的能力而言，通过饮水途径以每毫升10(6) CCID50的浓度接种疫苗与传统皮肤接种一样有效。2日龄时进行饮水接种并不比1日龄时接种更有效，并且口服疫苗给药比将疫苗加入饮水中效果更差。得出的结论是，如果疫苗含有足够高浓度的病毒，1日龄雏鸡可通过饮水途径接种鸡痘疫苗。
