Scott M G, Zachau H G, Nahm M H
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri.
Int Rev Immunol. 1992;9(1):45-55. doi: 10.3109/08830189209061782.
The V region repertoire of the human antibody response to the type b capsular polysaccharide of Haemophilus influenzae (Hib-PS) is being defined at the molecular level using antibodies purified from serum of immunized adults. The VH of this response is restricted to the VHIII subgroup while the VL can be divided into two categories. The most common VL, expressed in > 90% of adults and usually constituting the majority of a subjects anti-Hib-PS antibody response, is restricted to the product of a single V kappa II gene known as A2 that probably lacks somatic mutations. The product of the A2 gene is invariably joined to one of several J kappa products by an inserted arginine at the V kappa-J kappa junction. In contrast to the restricted nature of the dominant VL clonotype, the second category of VL constitutes a heterogeneous group of at least seven different VL gene products that often contain somatic mutations and generally exhibit crossreactivity with a related polysaccharide from E. coli. Elucidation of anti-Hib-PS V regions at the molecular level will permit examination of structure-function relationships among these clinically important antibodies and should make the V region repertoire to Hib-PS a useful model for studying human V gene responses.
利用从免疫成人血清中纯化的抗体，正在分子水平上确定人类对b型流感嗜血杆菌（Hib-PS）荚膜多糖抗体反应的V区库。这种反应的VH限于VHIII亚组，而VL可分为两类。最常见的VL在90%以上的成人中表达，通常构成受试者抗Hib-PS抗体反应的大部分，限于一个称为A2的单一VκII基因的产物，该基因可能缺乏体细胞突变。A2基因的产物总是通过在Vκ-Jκ连接处插入精氨酸与几种Jκ产物之一连接。与占主导地位的VL克隆型的受限性质相反，第二类VL构成了至少七种不同VL基因产物的异质组，这些产物通常含有体细胞突变，并且通常与来自大肠杆菌的相关多糖表现出交叉反应性。在分子水平上阐明抗Hib-PS V区将允许检查这些临床重要抗体之间的结构-功能关系，并应使针对Hib-PS的V区库成为研究人类V基因反应的有用模型。
