Gadkar Vijay, David-Schwartz Rakefet, Nagahashi Gerald, Douds David D, Wininger Smadar, Kapulnik Yoram
Department of Agronomy and Natural Resources, ARO, the Volcani Center, PO Box 6, Bet Dagan 50 250, Israel.
FEMS Microbiol Lett. 2003 Jun 27;223(2):193-8. doi: 10.1016/S0378-1097(03)00357-4.
Soluble factors released from roots of the pre-mycorrhizal infection (pmi) myc(-) tomato mutant M161 were analyzed and compared with normal wild-type released factors. Aseptic whole exudates from the M161 mutant retarded the proliferation of Glomus intraradices in vitro. When the whole exudate was further fractionated on a C18 SEPAK cartridge, the 50/70% methanol fraction showed an activity against hyphal tip growth of Gigaspora gigantea and Gl. intraradices. Preliminary characterization of the exudate suggests that the inhibitory moieties are heat labile, bind to PVPP (polyvinyl polypyrrolidone), and are not volatile. This is the first reported instance of the inhibition by a myc(-) plant being ascribed to inhibitory component(s) released in root exudate.
对菌根感染前（pmi）的myc（-）番茄突变体M161根系释放的可溶性因子进行了分析，并与正常野生型释放的因子进行了比较。M161突变体的无菌全分泌物在体外抑制了根内球囊霉的增殖。当全分泌物在C18 SEPAK柱上进一步分离时，50/70%甲醇部分显示出对巨型球囊霉和根内球囊霉菌丝顶端生长的活性。对分泌物的初步表征表明，抑制部分对热不稳定，与PVPP（聚乙烯聚吡咯烷酮）结合，且不挥发。这是首次报道myc（-）植物的抑制作用归因于根系分泌物中释放的抑制成分。
