Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK.
Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland.
Nat Plants. 2017 May 26;3:17073. doi: 10.1038/nplants.2017.73.
Most terrestrial plants, including crops, engage in beneficial interactions with arbuscular mycorrhizal fungi. Vital to the association is mutual recognition involving the release of diffusible signals into the rhizosphere. Previously, we identified the maize no perception 1 (nope1) mutant to be defective in early signalling. Here, we report cloning of ZmNope1 on the basis of synteny with rice. NOPE1 encodes a functional homologue of the Candida albicans N-acetylglucosamine (GlcNAc) transporter NGT1, and represents the first plasma membrane GlcNAc transporter identified from plants. In C. albicans, exposure to GlcNAc activates cell signalling and virulence. Similarly, in Rhizophagus irregularis treatment with rice wild-type but not nope1 root exudates induced transcriptome changes associated with signalling function, suggesting a requirement of NOPE1 function for presymbiotic fungal reprogramming.
大多数陆生植物，包括作物，与丛枝菌根真菌进行有益的相互作用。这种共生关系的关键是涉及到扩散信号释放到根际的相互识别。以前，我们发现玉米无感知 1（nope1）突变体在早期信号传递中存在缺陷。在这里，我们基于与水稻的同线性，报道了ZmNope1 的克隆。NOPE1 编码了 Candida albicans N-乙酰氨基葡萄糖（GlcNAc）转运蛋白 NGT1 的功能同源物，是植物中鉴定的第一个质膜 GlcNAc 转运蛋白。在 C. albicans 中，暴露于 GlcNAc 会激活细胞信号转导和毒力。同样，在 Rhizophagus irregularis 处理中，与水稻野生型相比，但 nope1 根分泌物处理诱导了与信号功能相关的转录组变化，表明 NOPE1 功能对于共生真菌重编程的需求。
