Li Fu Li, Xu Ping, Ma Cui Qing, Luo Lai Long, Wang Xiao Shan
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
FEMS Microbiol Lett. 2003 Jun 27;223(2):301-7. doi: 10.1016/S0378-1097(03)00397-5.
The dibenzothiophene (DBT) desulfurization pathway of a facultative thermophilic bacterium Mycobacterium sp. X7B was investigated. Metabolites were identified by gas chromatography-mass spectrometry, and the results showed that 2-hydroxybiphenyl, the end product of the previously reported sulfur-specific pathway (also called 4S pathway), was further converted to 2-methoxybiphenyl. This is the first strain to possess this ability and therefore, an extended 4S pathway was determined. In addition, the DBT-desulfurizing bacterium Mycobacterium sp. X7B was able to grow on DBT derivatives such as 4-methylDBT and 4,6-dimethylDBT. Resting cells could desulfurize diesel oil (total sulfur, 535 ppm) after hydrodesulfurization. GC flame ionization detection and GC atomic emission detection analyses were used to qualitatively evaluate the effect of Mycobacterium sp. X7B treatment on the content of the diesel oil. The total sulfur content of the diesel oil was reduced 86% using resting cell biocatalysts for 24 h at 45 degrees C.
对兼性嗜热细菌分枝杆菌属X7B的二苯并噻吩（DBT）脱硫途径进行了研究。通过气相色谱 - 质谱法鉴定代谢产物，结果表明，先前报道的硫特异性途径（也称为4S途径）的终产物2 - 羟基联苯进一步转化为2 - 甲氧基联苯。这是第一株具有这种能力的菌株，因此确定了一条扩展的4S途径。此外，DBT脱硫细菌分枝杆菌属X7B能够在DBT衍生物如4 - 甲基DBT和4,6 - 二甲基DBT上生长。加氢脱硫后的静息细胞能够对柴油（总硫含量为535 ppm）进行脱硫。采用气相色谱火焰离子化检测和气相色谱原子发射检测分析方法定性评估分枝杆菌属X7B处理对柴油含量的影响。在45℃下使用静息细胞生物催化剂24小时后，柴油的总硫含量降低了86%。
