Furuya T, Kirimura K, Kino K, Usami S
Department of Applied Chemistry, School of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
FEMS Microbiol Lett. 2001 Oct 16;204(1):129-33. doi: 10.1111/j.1574-6968.2001.tb10875.x.
Dibenzothiophene (DBT) derivatives can be detected in diesel oil following hydrodesulfurization treatment, and they are widely recognized as target compounds for more efficient desulfurization. The moderately thermophilic bacterium Mycobacterium phlei WU-F1 was isolated for its ability to grow at 50 degrees C in a medium with DBT as the sole source of sulfur. At 50 degrees C, resting cells of WU-F1 degraded 0.81 mM DBT within only 90 min to produce 2-hydroxybiphenyl as a desulfurized metabolite through the selective cleavage of carbon-sulfur bonds, and also degraded 0.81 mM of derivatives such as 2,8-dimethylDBT, 4,6-dimethylDBT and 3,4-benzoDBT within 8 h. In addition, the resting cells exhibited high DBT-desulfurizing ability over a wide temperature range from 20 to 50 degrees C. Because M. phlei WU-F1 possesses higher desulfurizing ability toward DBT and the derivatives over a wider temperature range than any other microorganisms previously reported, it may have useful practical applications for biodesulfurization.
在加氢脱硫处理后的柴油中可以检测到二苯并噻吩（DBT）衍生物，它们被广泛认为是更高效脱硫的目标化合物。中度嗜热细菌草分枝杆菌WU-F1因其能够在以DBT作为唯一硫源的培养基中于50℃生长的能力而被分离出来。在50℃下，WU-F1的静息细胞仅在90分钟内就能降解0.81 mM DBT，通过选择性断裂碳-硫键产生2-羟基联苯作为脱硫代谢产物，并且在8小时内还能降解0.81 mM的衍生物，如2,8-二甲基DBT、4,6-二甲基DBT和3,4-苯并DBT。此外，静息细胞在20至50℃的宽温度范围内表现出高DBT脱硫能力。由于草分枝杆菌WU-F1在比先前报道的任何其他微生物更宽的温度范围内对DBT及其衍生物具有更高的脱硫能力，它可能在生物脱硫方面具有有用的实际应用。
