Du Ning, Liu Xiang Y, Hew Choy Leong
Department of Physics, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
J Biol Chem. 2003 Sep 19;278(38):36000-4. doi: 10.1074/jbc.M305222200. Epub 2003 Jun 26.
The effect of antifreeze protein type III (one type of fish antifreeze protein) on ice crystallization was examined quantitatively based on a "micro-sized ice nucleation" technique. It was found for the first time that antifreeze proteins can inhibit the ice nucleation process by adsorbing onto both the surfaces of ice nuclei and dust particles. This leads to an increase of the ice nucleation barrier and the desolvation kink kinetics barrier, respectively. Based on the latest nucleation model, the increases in the ice nucleation barrier and the kink kinetics barrier were measured. This enables us to quantitatively examine the antifreeze mechanism of antifreeze proteins for the first time.
基于“微尺寸冰核形成”技术，对III型抗冻蛋白（一种鱼类抗冻蛋白）对冰结晶的影响进行了定量研究。首次发现抗冻蛋白可通过吸附在冰核和尘埃颗粒表面来抑制冰核形成过程。这分别导致冰核形成势垒和去溶剂化扭结动力学势垒增加。基于最新的成核模型，测量了冰核形成势垒和扭结动力学势垒的增加。这使我们首次能够定量研究抗冻蛋白的抗冻机制。
