Du Ning, Liu Xiang Y, Hew Choy L
Biophysics & Micro/nanostructures Lab, Department of Physics, National University of Singapore, 10 Kent Ridge Crescent, Singapore 117542.
J Phys Chem B. 2006 Oct 19;110(41):20562-7. doi: 10.1021/jp061969y.
Antifreeze protein type III aggregates once the concentration exceeds a critical value, the so-called critical aggregation concentration (CAC). It was found for the first time that the aggregation of antifreeze protein exerts a direct impact on the antifreeze efficiency. It follows from our measurements that the AFP III above CAC will enhance the antifreeze activity because of the increase of the kink kinetics barrier of surface integration. This is attributed to the optimal packing of AFP III molecules on the surface of the ice nucleus as well as ice crystals above CAC. This study will extend our understanding of the antifreeze mechanism of antifreeze protein monomers as well as antifreeze aggregates on ice nucleation and shed light on the selection of antifreeze agents.
III型抗冻蛋白一旦浓度超过临界值（即所谓的临界聚集浓度，CAC）就会发生聚集。首次发现抗冻蛋白的聚集对抗冻效率有直接影响。我们的测量结果表明，高于CAC的AFP III会增强抗冻活性，这是因为表面整合的扭结动力学障碍增加。这归因于高于CAC时AFP III分子在冰核以及冰晶表面的最佳堆积。这项研究将扩展我们对抗冻蛋白单体以及抗冻聚集体在冰核形成方面的抗冻机制的理解，并为抗冻剂的选择提供启示。
