Ho Yu- Hsiang, Wu Hsin- Lung, Wu Shou- Mei, Chen Su- Hwei, Kou Hwang- Shang
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, 807, Kaohsiung, Taiwan.
Anal Bioanal Chem. 2003 Jul;376(6):859-63. doi: 10.1007/s00216-003-2015-x. Epub 2003 Jun 26.
A simple capillary zone electrophoresis method was developed for the quantitative enantiomeric analysis of piperazine antihistamines with teratogenic suspicion in animals. Enantioseparation of chlorcyclizine, hydroxyzine, and meclizine was performed in glycine buffer (0.6 mol L(-1); pH 3.00) with sulfated beta-cyclodextrin (5 mg mL(-1)) as a chiral selector; and the separated drugs were monitored by ultra-violet detector. The lower quantitation of the individual enantiomer is attainable at 10 micro mol L(-1), using an achiral piperazine drug (cyclizine) as internal standard. The method is simple and rapid with a short run time (<5 min) for the analysis of chlorcyclizine, hydroxyzine or meclizine enantiomers.
建立了一种简单的毛细管区带电泳方法，用于对动物体内具有致畸嫌疑的哌嗪类抗组胺药进行对映体定量分析。在含有硫酸化β-环糊精（5 mg mL(-1)）作为手性选择剂的甘氨酸缓冲液（0.6 mol L(-1)；pH 3.00）中对氯环利嗪、羟嗪和氯苯甲嗪进行对映体分离；分离出的药物用紫外检测器进行监测。以非手性哌嗪药物（赛克利嗪）作为内标，各对映体的最低定量限可达10 μmol L(-1)。该方法简单快速，分析氯环利嗪、羟嗪或氯苯甲嗪对映体的运行时间较短（<5分钟）。
