Verboon-Maciolek M A, Nijhuis M, van Loon A M, van Maarssenveen N, van Wieringen H, Pekelharing-Berghuis M A, Krediet T G, Gerards L J, Fleer A, Diepersloot R J A, Thijsen S F T
Department of Neonatology, Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Medical Center, Utrecht, The Netherlands.
Clin Infect Dis. 2003 Jul 1;37(1):1-6. doi: 10.1086/375222. Epub 2003 Jun 20.
During summer and fall, enterovirus infections are responsible for a considerable proportion of hospitalizations of young infants. We prospectively studied the incidence of enterovirus infections via real-time polymerase chain reaction (PCR) in blood, feces, and cerebrospinal fluid samples from infants <or=60 days old who had received a clinical diagnosis of sepsis. Forty-five patients were included: 19 were admitted to the pediatric wards of 2 general hospitals, and 26 had been hospitalized since birth in the neonatal intensive care unit (NICU) of a tertiary care hospital. None of the NICU patients developed enteroviral disease. In contrast, an enterovirus was detected in 11 (58%) of the patients admitted to the 2 general hospitals, 10 of whom (53%) showed evidence of systemic infection. Enterovirus infections are an important cause of sepsis in infants admitted to the hospital. Real-time PCR in serum was a rapid and sensitive method for diagnosis of enterovirus infection.
在夏季和秋季，肠道病毒感染导致相当一部分幼儿住院。我们对临床诊断为败血症的60日龄及以下婴儿的血液、粪便和脑脊液样本进行实时聚合酶链反应（PCR），前瞻性研究肠道病毒感染的发病率。共纳入45例患者：19例入住2家综合医院的儿科病房，26例自出生起就入住一家三级医院的新生儿重症监护病房（NICU）。NICU的患者均未发生肠道病毒疾病。相比之下，在入住2家综合医院的患者中，11例（58%）检测到肠道病毒，其中10例（53%）有全身感染的证据。肠道病毒感染是住院婴儿败血症的重要原因。血清中的实时PCR是诊断肠道病毒感染的快速、灵敏方法。
