Mori Masanobu, Tanaka Kazuhiko, Helaleh Murad I H, Xu Qun, Ikedo Mikaru, Ogura Yutaka, Sato Shinji, Hu Wenzhi, Hasebe Kiyoshi, Haddad Paul R
National Institute of Advanced Industrial Science and Technology at Seto, 110 Nishiibara-cho, Seto, Aichi 489-0884, Japan.
J Chromatogr A. 2003 May 16;997(1-2):219-24. doi: 10.1016/s0021-9673(03)00631-9.
The simultaneous ion-exclusion/cation-exchange separation column packed with a polymethacrylate-based weakly acidic cation-exchange resin of 3 microm particle size was used to achieve the simultaneous high-speed separation of anions and cations (Cl(-), NO3(-), SO4(2-), Na(+), K(+), NH4(+), Ca(2+) and Mg(2+)) commonly found in environmental samples. The high-speed simultaneous separation is based on a combination of the ion-exclusion mechanism for the anions and the cation-exchange mechanism for cations. The complete separation of the anions and cations was achieved in 5 min by elution with 15 mM tartaric acid-2.5 mM 18-crown-6 at a flow-rate of 1.5 ml/min. Detection limits at S/N=3 ranged from 0.36 to 0.68 microM for anions and 0.63-0.99 microM for cations. This method has been applied to the simultaneous determination of anions and cations in several environmental waters with satisfactory results.
采用填充有粒径为3微米的聚甲基丙烯酸酯基弱酸性阳离子交换树脂的同时离子排斥/阳离子交换分离柱，实现了环境样品中常见阴离子和阳离子（Cl(-)、NO3(-)、SO4(2-)、Na(+)、K(+)、NH4(+)、Ca(2+)和Mg(2+)）的同时高速分离。这种高速同时分离基于阴离子的离子排斥机制和阳离子的阳离子交换机制的结合。通过以1.5 ml/min的流速用15 mM酒石酸 - 2.5 mM 18 - 冠 - 6洗脱，在5分钟内实现了阴离子和阳离子的完全分离。信噪比S/N = 3时，阴离子的检测限为0.36至0.68 microM，阳离子的检测限为0.63 - 0.99 microM。该方法已应用于几种环境水样中阴离子和阳离子的同时测定，结果令人满意。
