Sawada K, Agata K, Eguchi G
Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.
Exp Eye Res. 1992 Dec;55(6):879-87. doi: 10.1016/0014-4835(92)90014-j.
One alpha B- and three different beta-crystallin cDNA clones were isolated from a chicken lens cDNA library by using anti-crystallin antibodies. The sequence of alpha B-crystallin cDNA showed more than 70% homology with exons of alpha B-crystallin genes of the human and hamster. Two beta-crystallin cDNAs showed almost identical sequences with previously reported chicken beta B1- and beta A3/A1-crystallin genes. The remainder showed 80% homology of sequence with bovine beta B2-crystalline cDNA. Using these newly cloned cDNAs, in addition to cDNAs of alpha A- and delta-crystallin, we examined the expression pattern of these crystallins in the process of lentoidogenesis of cultured lens epithelial cells of the chicken. All crystallins except beta-crystallins were expressed through the period of cell culture, but three beta-crystallins were expressed only after the confluent stage. These results suggest that: (1) alpha A-, alpha B- and delta-crystallin cDNAs can be used to detect differentiation of the lens epithelial cell; and (2) beta-crystallin cDNAs are superior in the detection of chicken lens fibre differentiation in vitro to delta-crystallin cDNA, which is ectopically expressed by various non-lenticular tissues.
利用抗晶状体蛋白抗体从鸡晶状体cDNA文库中分离出一个αB - 晶状体蛋白和三个不同的β - 晶状体蛋白cDNA克隆。αB - 晶状体蛋白cDNA序列与人及仓鼠αB - 晶状体蛋白基因的外显子显示出超过70%的同源性。两个β - 晶状体蛋白cDNA与先前报道的鸡βB1 - 和βA3/A1 - 晶状体蛋白基因显示出几乎相同的序列。其余的与牛βB2 - 晶状体蛋白cDNA显示出80%的序列同源性。利用这些新克隆的cDNA，以及αA - 和δ - 晶状体蛋白的cDNA，我们研究了这些晶状体蛋白在鸡培养晶状体上皮细胞晶状体样体形成过程中的表达模式。除β - 晶状体蛋白外，所有晶状体蛋白在细胞培养期间均有表达，但三种β - 晶状体蛋白仅在汇合期后才表达。这些结果表明：（1）αA - 、αB - 和δ - 晶状体蛋白cDNA可用于检测晶状体上皮细胞的分化；（2）在体外检测鸡晶状体纤维分化方面，β - 晶状体蛋白cDNA优于δ - 晶状体蛋白cDNA，后者在各种非晶状体组织中异位表达。
