用于原核生物鉴定和特征描述的荧光原位杂交技术。

Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes.

作者信息

Wagner Michael, Horn Matthias, Daims Holger

机构信息

Department of Microbial Ecology, Institute of Ecology and Conservation Biology, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.

出版信息

Curr Opin Microbiol. 2003 Jun;6(3):302-9. doi: 10.1016/s1369-5274(03)00054-7.

DOI:10.1016/s1369-5274(03)00054-7

PMID:12831908

Abstract

Fluorescence in situ hybridisation with rRNA-targeted nucleic acid probes can be used to directly identify microorganisms within complex samples in a few hours and therefore has widespread application in environmental and medical microbiology. The past year has seen significant methodological improvements in fluorescence in situ hybridisation, as well as in the combination of this method with other techniques for inferring functional traits of microorganisms within their environment.

摘要

使用针对rRNA的核酸探针进行荧光原位杂交，可在数小时内直接鉴定复杂样品中的微生物，因此在环境微生物学和医学微生物学中有着广泛应用。过去一年里，荧光原位杂交技术在方法上有了显著改进，该方法与其他用于推断微生物在其环境中的功能特性的技术相结合也有了显著进展。