Černohorská L, Dolinková L
Epidemiol Mikrobiol Imunol. 2017 Summer;66(2):86-90.
The diagnosis of bacterial agents of sepsis from blood cultures is crucial for the subsequent treatment of this condition. The aim of this study was to compare Gram stain, culture, and biochemical identification (conventional methods) and fluorescence in-situ hybridisation (FISH) that detects microorganisms from positive blood cultures using specific probes. Another aim was to evaluate the potential of this method for use in clinical practice. Altogether 71 samples of positive blood cultures were tested by FISH. Blood cultures were also processed in the conventional way using the BACTEC analyser. The bacteria recovered were inoculated on solid media and then identified biochemically. The results obtained by the conventional methods and HemoFISH were not 100% concordant. The sensitivity of HemoFISH was 90.1%. Very good results were achieved for staphylococci and enterobacteria. FISH identification failed in three cases because the hybridization probes were not able to bind to bacterial rRNA. The FISH bacterial identification is faster than the conventional methods, but should be confirmed by the latter.
通过血培养诊断败血症的细菌病原体对于该病症的后续治疗至关重要。本研究的目的是比较革兰氏染色、培养和生化鉴定（传统方法）以及使用特异性探针从阳性血培养物中检测微生物的荧光原位杂交（FISH）。另一个目的是评估该方法在临床实践中的应用潜力。总共71份阳性血培养样本通过FISH进行检测。血培养物也使用BACTEC分析仪以传统方式进行处理。将分离出的细菌接种在固体培养基上，然后进行生化鉴定。传统方法和HemoFISH获得的结果并非100%一致。HemoFISH的灵敏度为90.1%。葡萄球菌和肠杆菌的检测结果非常好。有3例FISH鉴定失败，原因是杂交探针无法与细菌rRNA结合。FISH细菌鉴定比传统方法更快，但应由传统方法进行确认。
