F98大鼠胶质瘤中氟酪氨酸和蛋氨酸摄取的比较。
Comparison of fluorotyrosines and methionine uptake in F98 rat gliomas.
作者信息
Langen Karl-Josef, Jarosch Michael, Mühlensiepen Heinz, Hamacher Kurt, Bröer Stefan, Jansen Paul, Zilles Karl, Coenen Heinz H
机构信息
Institute of Medicine, Research Centre Jülich, FRG, Australia.
出版信息
Nucl Med Biol. 2003 Jul;30(5):501-8. doi: 10.1016/s0969-8051(03)00023-4.
The transport mechanisms of O-(2-[(18)F]fluoroethyl)-L-tyrosine (FET) and 2-[(18)F]fluoro-L-tyrosine (FTyr) were compared to those of [(3)H]-Methyl-L-methionine (MET) in F98 rat glioma cells in vitro and by tumor imaging by ex vivo dual tracer autoradiography in F98 rat gliomas. Both, FET and FTyr exhibited similar transport characteristics in F98 glioma cells compared to MET, i.e. mainly a sodium dependent transport similar to system B(0,+) and sodium independent transport via system L. Radioactivity of FET in the acid precipitable fraction was <1% after 120 min incubation time while FTyr and MET exhibited a 15-18% incorporation into proteins. Comparison of FET and FTyr with MET uptake in F98 rat gliomas demonstrated a significant correlation of tumor to brain ratios and a similar intratumoral tracer distribution pattern.
在体外培养的F98大鼠胶质瘤细胞中，以及通过对F98大鼠胶质瘤进行离体双示踪放射自显影肿瘤成像，比较了O-(2-[(18)F]氟乙基)-L-酪氨酸（FET）和2-[(18)F]氟-L-酪氨酸（FTyr）与[(3)H]-甲基-L-蛋氨酸（MET）的转运机制。与MET相比，FET和FTyr在F98胶质瘤细胞中表现出相似的转运特征，即主要是类似于系统B(0,+)的钠依赖性转运以及通过系统L的钠非依赖性转运。孵育120分钟后，酸沉淀部分中FET的放射性小于1%，而FTyr和MET在蛋白质中的掺入率为15 - 18%。比较FET和FTyr与MET在F98大鼠胶质瘤中的摄取情况，结果显示肿瘤与脑的比值存在显著相关性，且瘤内示踪剂分布模式相似。
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