Inadera H, Tashiro J, Okubo Y, Ishikawa Y, Shirai K, Saito Y, Yoshida S
Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
Scand J Clin Lab Invest. 1992 Dec;52(8):797-802. doi: 10.3109/00365519209088383.
The mechanism regulating lipoprotein lipase (LPL) expression in adipose tissue was examined in rats in the conditions of different calorie intakes with and without streptozotocin-induced (STZ-) diabetes. The LPL activity released from adipose tissue was greater with the higher calorie intake (20 g of normal chow diet per day) than with the lower calorie intake (13 g of normal chow diet per day), and was greater in normal rats than in STZ-diabetic rats. The LPL activity was proportional to the serum insulin level in all conditions. Dot-blot analysis showed that the amount of LPL mRNA in adipose tissue was increased by the higher calorie diet and that the increase was less in the diabetic state. Expression of mRNA was also nearly parallel with the serum insulin level. LPL activity released from the heart was not affected by either the calorie intake or the diabetic state. These results suggest that the mechanisms of LPL expression in adipose tissue and the heart are different, and that LPL expression in adipose tissue was closely dependent on the insulin level.
在有或没有链脲佐菌素诱导的糖尿病（STZ-糖尿病）的情况下，在不同卡路里摄入量的大鼠中研究了调节脂肪组织中脂蛋白脂肪酶（LPL）表达的机制。与较低卡路里摄入量（每天13克正常饲料）相比，较高卡路里摄入量（每天20克正常饲料）时，脂肪组织释放的LPL活性更高，并且正常大鼠中的LPL活性高于STZ-糖尿病大鼠。在所有情况下，LPL活性均与血清胰岛素水平成正比。斑点印迹分析表明，较高卡路里饮食可增加脂肪组织中LPL mRNA的量，而在糖尿病状态下这种增加较少。mRNA的表达也与血清胰岛素水平几乎平行。心脏释放的LPL活性不受卡路里摄入量或糖尿病状态的影响。这些结果表明，脂肪组织和心脏中LPL表达的机制不同，并且脂肪组织中LPL的表达密切依赖于胰岛素水平。
