Feichtinger H, Kaaya E E, Li S L, Putkonen P, Grünewald K, Weyrer K, Offner F, Biberfeld G, Biberfeld P
Institut für Pathologie, Universität Innsbruck.
Verh Dtsch Ges Pathol. 1992;76:189-93.
In a series of 33 cynomolgus monkeys (Macaca fascicularis) experimentally infected with Simian Immunodeficiency virus (SIV), strain smm3, 13 animals developed malignant Non-Hodgkin lymphomas. These lymphomas presented with unusual primary manifestations like in the orbita, testes, and brain. The morphological features and immunophenotyping identified the tumors as high malignant B-cell lymphomas. In all tumors as well as in tumor-derived cell lines a cynomolgus B-lymphotropic herpes virus (CBLV) with structural homogeneity to the Epstein-Barr virus (EBV) could be demonstrated by Southern blotting with EBV-specific probes. The lymphoma cells also expressed CBLV-associated nuclear antigens involved in B-cell transformation crossreacting with EBNA-specific human sera and monoclonal antibodies. Ig-gene rearrangement studies revealed clonal populations, however, no translocations of the c-myc oncogene could be detected. The lymphomas developing with high frequency in SIV-induced immunodeficiency resemble a major subtype of human EBV-associated AIDS lymphomas. This animal model can therefore be used to further elucidate interactions of HIV and EBV in AIDS-related lymphomagenesis.
在一组33只经实验感染猿猴免疫缺陷病毒（SIV）smm3株的食蟹猴（猕猴）中，有13只动物发生了恶性非霍奇金淋巴瘤。这些淋巴瘤呈现出不寻常的主要表现，如眼眶、睾丸和脑部。形态学特征和免疫表型分析确定这些肿瘤为高恶性B细胞淋巴瘤。通过用EBV特异性探针进行Southern印迹分析，在所有肿瘤以及肿瘤衍生细胞系中都可证实一种与爱泼斯坦-巴尔病毒（EBV）结构同源的食蟹猴B淋巴细胞嗜性疱疹病毒（CBLV）。淋巴瘤细胞还表达与EBNA特异性人血清和单克隆抗体发生交叉反应的、参与B细胞转化的CBLV相关核抗原。Ig基因重排研究显示为克隆性群体，然而，未检测到c-myc癌基因的易位。在SIV诱导的免疫缺陷中高频发生的淋巴瘤类似于人类EBV相关艾滋病淋巴瘤的一种主要亚型。因此，这个动物模型可用于进一步阐明HIV和EBV在艾滋病相关淋巴瘤发生中的相互作用。
