Ohshima K, Suzumiya J, Tasiro K, Mukai Y, Tanaka T, Kato A, Kikuchi M
Department of Pathology, School of Medicine, Fukuoka University, Japan.
Am J Hematol. 1996 May;52(1):21-8. doi: 10.1002/(SICI)1096-8652(199605)52:1<21::AID-AJH4>3.0.CO;2-8.
Sixty cases of B-cell nodal non-Hodgkin's malignant lymphoma (B-ML), and 46 cases of T-cell nodal lymphoma (T-ML) were surveyed for Epstein-Barr virus (EBV) genomes, RNA, and associated proteins. We used a Southern blot analysis, polymerase chain reaction (PCR), and EBV-encoded small RNA-1 (EBER-1) in situ hybridization to investigate the presence of EBV. We performed an immunohistochemical study on EBV-related oncoproteins, such as EBV-determined nuclear antigen-2 (EBNA-2), latent membrane protein (LMP), and viral interleukin-10 (vIL-10). In addition, we also analyzed the terminal repetitive sequence of EBV (EBV-TR) to investigate the EBV-infected cell clonality. Non-Hodgkin's lymphomas were grouped into three types by number of EBV-infected cells: I) almost all lymphoma cells showed an EBV presence; II) some scattered lymphoma cells showed an EBV presence; and III) only a few cells showed such a presence, which was probably due to a latent EBV infection. In 25 of 60 B-MLs, EBV-infected cells were found; 7 were type I, 1 was type II, and 17 were type III. In 27 of 46 T-MLs, EBV-infected cells were found; no cases were type I, 5 cases were type II, and 22 cases were type III. Seven B-MLs and 3 T cell lymphomas showed clonal TR bands. Expression of EBNA-2 was found in only three B-MLs, whereas LMP was seen in four B-MLs and six T-MLs. All EBNA-2/LMP-positive cases showed an EBV presence. In B-MLs, expression of EBNA-2 and LMP was detected in almost all lymphoma cells; in T-MLs, however, LMP was found in only a small portion of the lymphoma cells. Expression of IL-10 was closely associated with LMP. In summary, it was thus speculated that EBV infection was associated with the various states of lymphomagenesis.
对60例B细胞结节性非霍奇金恶性淋巴瘤（B-ML）和46例T细胞结节性淋巴瘤（T-ML）进行了爱泼斯坦-巴尔病毒（EBV）基因组、RNA及相关蛋白的检测。我们采用Southern印迹分析、聚合酶链反应（PCR）以及EBV编码的小RNA-1（EBER-1）原位杂交来研究EBV的存在情况。我们对EBV相关的癌蛋白进行了免疫组织化学研究，如EBV限定核抗原-2（EBNA-2）、潜伏膜蛋白（LMP）和病毒白细胞介素-10（vIL-10）。此外，我们还分析了EBV的末端重复序列（EBV-TR）以研究EBV感染细胞的克隆性。非霍奇金淋巴瘤根据EBV感染细胞数量分为三种类型：I）几乎所有淋巴瘤细胞均显示有EBV存在；II）一些散在的淋巴瘤细胞显示有EBV存在；III）仅有少数细胞显示有EBV存在，这可能是由于EBV潜伏感染所致。在60例B-ML中，发现25例有EBV感染细胞；7例为I型，1例为II型，17例为III型。在46例T-ML中，发现27例有EBV感染细胞；无I型病例，5例为II型，22例为III型。7例B-ML和3例T细胞淋巴瘤显示出克隆性TR条带。仅在3例B-ML中发现EBNA-2表达，而在4例B-ML和6例T-ML中可见LMP表达。所有EBNA-2/LMP阳性病例均显示有EBV存在。在B-ML中，几乎所有淋巴瘤细胞均检测到EBNA-2和LMP表达；然而，在T-ML中，仅在一小部分淋巴瘤细胞中发现LMP表达。IL-10的表达与LMP密切相关。总之，由此推测EBV感染与淋巴瘤发生的不同状态有关。
