古菌依赖ADP的磷酸果糖激酶：表达、纯化、结晶及初步晶体学分析。
Archaeal ADP-dependent phosphofructokinase: expression, purification, crystallization and preliminary crystallographic analysis.
作者信息
Jeong Jong-Jin, Fushinobu Shinya, Ito Sohei, Shoun Hirofumi, Wakagi Takayoshi
机构信息
Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1327-9. doi: 10.1107/s0907444903010631. Epub 2003 Jun 27.
Thermococcus litoralis uses a modified Embden-Meyerhof pathway. Its phosphofructokinase (TLPFK) catalyses the phosphorylation of fructose-6-phosphate by ADP, but not by ATP, in the presence of Mg(2+), yielding fructose-1,6-bisphosphate. The gene encoding TLPFK was cloned and overexpressed in Escherichia coli. Recombinant TLPFK consists of a dimer with a 52 kDa subunit. The native crystals belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 85.41, c = 163.93 A, and diffract to beyond 2.6 A resolution. The TLPFK structure was preliminarily analyzed by means of multiple isomorphous replacement with four heavy-atom derivatives.
嗜热栖热菌采用一种改良的糖酵解途径。其磷酸果糖激酶（TLPFK）在Mg(2+)存在的情况下，催化由ADP而非ATP将6-磷酸果糖磷酸化，生成1,6-二磷酸果糖。编码TLPFK的基因被克隆并在大肠杆菌中过表达。重组TLPFK由一个含有52 kDa亚基的二聚体组成。天然晶体属于空间群P4(1)2(1)2，晶胞参数a = b = 85.41，c = 163.93 Å，并且衍射分辨率超过2.6 Å。通过使用四种重原子衍生物的多同晶置换法对TLPFK结构进行了初步分析。
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