Das Satyabrata, Kumar Parimal, Bhor Vikrant, Surolia A, Vijayan M
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):65-7. doi: 10.1107/S1744309104028040. Epub 2004 Nov 9.
Pantothenate kinase is an essential enzyme in the bacterial life cycle. It catalyzes the phosphorylation of pantothenate (vitamin B5) to 4'-phosphopantothenate, the first step in the coenzyme A biosynthetic pathway. The enzyme from Mycobacterium tuberculosis, MW 35.7 kDa, has been cloned, expressed, purified and crystallized in two different trigonal crystal forms, both belonging to space group P3(1)21. Two complete data sets of resolution 2.5 A (form I) and 2.9 A (form II) from crystals with unit-cell parameters a = b = 78.3, c = 115.45 A and a = b = 107.63, c = 89.85 A, respectively, were collected at room temperature on a home X-ray source. Structures of both crystal forms were solved for one subunit in the asymmetric unit by molecular replacement.
泛酸激酶是细菌生命周期中的一种必需酶。它催化泛酸（维生素B5）磷酸化生成4'-磷酸泛酸，这是辅酶A生物合成途径的第一步。来自结核分枝杆菌的该酶分子量为35.7 kDa，已被克隆、表达、纯化，并以两种不同的三角晶体形式结晶，二者均属于空间群P3(1)21。分别从晶胞参数为a = b = 78.3、c = 115.45 Å（晶型I）和a = b = 107.63、c = 89.85 Å（晶型II）的晶体中，在室温下于家用X射线源上收集了分辨率为2.5 Å（晶型I）和2.9 Å（晶型II）的两个完整数据集。通过分子置换法解析了不对称单元中一个亚基的两种晶体形式的结构。
