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假结核耶尔森氏菌衍生促细胞分裂剂的结晶及初步X射线分析

Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen.

作者信息

Donadini Roberta, Wåhlberg Malin, Kohsaka Takao, Ito Yasuhiko, Fields Barry A

机构信息

School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia.

出版信息

Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1330-2. doi: 10.1107/s0907444903010710. Epub 2003 Jun 27.

DOI:10.1107/s0907444903010710
PMID:12832802
Abstract

Yersinia pseudotuberculosis-derived mitogen (YPM), a superantigen with no amino-acid sequence similarity to other known superantigens, has been crystallized by the sitting-drop vapour-diffusion method. The crystals belong to space group C2, with unit-cell parameters a = 138.67, b = 78.66, c = 32.91 A, beta = 91.97 degrees. A native data set has been collected to a resolution of 1.8 A using synchrotron radiation. Self-rotation function calculations suggest the presence of three molecules in the asymmetric unit, corresponding to a solvent content of 45%.

摘要

假结核耶尔森氏菌衍生促细胞分裂剂(YPM)是一种与其他已知超抗原无氨基酸序列相似性的超抗原,已通过坐滴气相扩散法结晶。晶体属于空间群C2,晶胞参数为a = 138.67、b = 78.66、c = 32.91 Å,β = 91.97°。利用同步辐射已收集到分辨率为1.8 Å的天然数据集。自旋转函数计算表明不对称单元中存在三个分子,溶剂含量为45%。

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