Nakamura T, Ebihara I, Nagaoka I, Takahashi T, Tomino Y, Koide H
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Am J Nephrol. 1992;12(5):292-302. doi: 10.1159/000168463.
We investigated insulin-like growth factor (IGF)-I and -II mRNA expression in peripheral blood mononuclear cells (PBMC) and T cells obtained from 31 patients with IgA nephropathy (IgAN), 43 patients with other types of glomerulonephritis and 16 health age-matched controls. The majority of patients with IgAN showed elevated IGF-I and -II mRNA expression in PBMC, while no IGF-I and -II mRNA expression was detected in PBMC obtained from patients with other types of glomerulonephritis or normal controls. In T cells obtained from IgAN, other types of glomerulonephritis and normal controls, however, IGF-I and -II mRNA expression was not detected. A positive correlation was noted between IGF-I and -II mRNA levels and urinary protein excretion. IGF-I and -II mRNA expression also correlated with the histopathological findings in the renal tissue of patients with IgAN. Sixty-nine percent of patients with more than 1.0 g/day proteinuria showed strong [more than (++)] IGF-I and -II mRNA expression in their PBMC. Eighty-one and 76% of patients with grade III and IV histopathological findings, respectively, showed strong IGF-I and -II gene expression in their PBMC. We also studied the clinical course of 11 patients with IgAN during hospitalization. The IGF-I and -II mRNA levels in these patients decreased gradually, as did proteinuria, after treatment. These studies suggest that abnormal regulation of IGF-I and -II gene expression in PBMC may be associated with the progression of IgAN and may be useful as an indicator of disease activity.
我们研究了31例IgA肾病（IgAN）患者、43例其他类型肾小球肾炎患者和16例年龄匹配的健康对照者外周血单个核细胞（PBMC）和T细胞中胰岛素样生长因子（IGF）-I和-II mRNA的表达。大多数IgAN患者的PBMC中IGF-I和-II mRNA表达升高，而在其他类型肾小球肾炎患者或正常对照者的PBMC中未检测到IGF-I和-II mRNA表达。然而，在IgAN、其他类型肾小球肾炎患者及正常对照者的T细胞中均未检测到IGF-I和-II mRNA表达。IGF-I和-II mRNA水平与尿蛋白排泄呈正相关。IGF-I和-II mRNA表达也与IgAN患者肾组织的组织病理学结果相关。69%蛋白尿超过1.0 g/天的患者PBMC中显示强（超过++）IGF-I和-II mRNA表达。分别有81%和76%组织病理学为III级和IV级的患者PBMC中显示强IGF-I和-II基因表达。我们还研究了11例IgAN患者住院期间的临床病程。治疗后，这些患者的IGF-I和-II mRNA水平逐渐下降，蛋白尿也逐渐减少。这些研究表明，PBMC中IGF-I和-II基因表达的异常调节可能与IgAN的进展有关，并且可能作为疾病活动的一个指标。
