Nakamura T, Ebihara I, Osada S, Takahashi T, Tomino Y, Okano K, Naruto M, Koide H
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Clin Nephrol. 1993 Apr;39(4):183-91.
We investigated mRNA expression for medullasin (an inflammatory serine protease in bone marrow cells) in peripheral blood mononuclear cells (PBMC) obtained from 36 patients with primary IgA nephropathy (IgAN), 30 patients with other types of primary glomerular disease, 18 patients with secondary IgA nephritis including lupus nephritis and hepatic glomerulosclerosis and 24 healthy age-matched controls. The majority of patients with IgAN (86%) showed elevated medullasin expression in PBMC, while no medullasin mRNA expression was detected in PBMC obtained from patients with other types of primary glomerular disease, secondary IgA nephritis or normal healthy controls. A positive correlation was noted between mRNA levels and urinary protein excretion. The medullasin mRNA expression in PBMC also correlated with the severity of the histopathologic changes in renal tissue obtained from patients with IgAN. All the patients with severe proteinuria (more than 3.0 g/day) showed strong [more than (++)] medullasin mRNA expression in their PBMC. In addition, all the patients with more than (++) medullasin mRNA expression are grade III or IV histopathological findings. These studies suggest that abnormally regulated medullasin gene expression in PBMC may be associated with the progression of primary IgAN.
我们研究了从36例原发性IgA肾病（IgAN）患者、30例其他类型原发性肾小球疾病患者、18例继发性IgA肾炎（包括狼疮性肾炎和肝性肾小球硬化症）患者以及24名年龄匹配的健康对照者获取的外周血单个核细胞（PBMC）中髓拉辛（骨髓细胞中的一种炎性丝氨酸蛋白酶）的mRNA表达情况。大多数IgAN患者（86%）的PBMC中髓拉辛表达升高，而在其他类型原发性肾小球疾病患者、继发性IgA肾炎患者或正常健康对照者的PBMC中未检测到髓拉辛mRNA表达。mRNA水平与尿蛋白排泄之间存在正相关。PBMC中髓拉辛mRNA表达也与IgAN患者肾组织的组织病理学改变严重程度相关。所有重度蛋白尿患者（超过3.0 g/天）的PBMC中均显示强烈的[超过（++）]髓拉辛mRNA表达。此外，所有髓拉辛mRNA表达超过（++）的患者组织病理学表现均为III级或IV级。这些研究表明，PBMC中髓拉辛基因表达异常调节可能与原发性IgA肾病的进展有关。
