Rosales-Cortes M, Peregrina-Sandoval J, Bañuelos-Pineda J, Castellanos-Martínez E E, Gómez-Pinedo U A, Albarrán-Rodríguez E
Laboratorio de Morfofisiología, Departamento de Medicina Veterinaria. Centro Universitario de Ciencias Biologicas y Agropecuarias, Universidad de Guadalajara, Mexico.
Rev Neurol. 2003;36(12):1137-41.
Injuries to peripheral nerves can have different causes and may lead to disorders affecting mobility, sensitivity and loss of motor function as they progress. Weiss, in 1944, introduced tubulisation to promote the regeneration of a sectioned nerve. In this study the biomaterial Chitosan was used to induce and stimulate the regeneration of the sciatic nerve in dogs. At the same time, we took advantage of the characteristics offered by Chitosan to include the neurosteroid progesterone in its matrix, as a promoter of axonal growth. AIMS. The aim of our study was to determine the degree of regeneration of the sciatic nerve in dogs when axotomised tubulised with a Chitosan prosthesis steeped in the neurosteroid progesterone.
Young adult female dogs were used to evaluate the regeneration of the sciatic nerve induced at a standard of 15 mm; regeneration was determined by means of an axonal growth chamber. Nerve growth was studied through histological analysis and by electron microscope.
The statistical analysis showed that there were no significant differences in the number of myelinated fibres between the experimental groups. The electron microscope images of the transmission in the regenerated nerves in the groups that were tubulised with Chitosan, with and without neurosteroid preloading, revealed an advanced regenerative process. This was evidenced by the fact that collagen fibres, elastin, Schwann cells and both myelinated and non myelinated fibres were observed in all cases.
The regeneration of axotomised, tubulised nerves was achieved regardless of the treatment that was applied. The distal nerve segment that was analysed revealed a similar structure to that of a normal nerve.
周围神经损伤可能有不同原因，随着病情发展，可能导致影响活动能力、感觉和运动功能丧失的病症。1944年，魏斯引入了套管法来促进离断神经的再生。在本研究中，生物材料壳聚糖被用于诱导和刺激犬坐骨神经的再生。同时，我们利用壳聚糖的特性，将神经甾体孕酮纳入其基质中，作为轴突生长的促进剂。目的。我们研究的目的是确定用浸泡在神经甾体孕酮中的壳聚糖假体进行轴突切断套管化后犬坐骨神经的再生程度。
使用年轻成年雌性犬评估以15毫米标准诱导的坐骨神经再生；通过轴突生长室确定再生情况。通过组织学分析和电子显微镜研究神经生长情况。
统计分析表明，各实验组之间有髓纤维数量无显著差异。用壳聚糖进行套管化处理的组，无论是否预先加载神经甾体，其再生神经中传输的电子显微镜图像均显示出先进的再生过程。所有病例中均观察到胶原纤维、弹性蛋白、施万细胞以及有髓和无髓纤维，这证明了这一点。
无论采用何种治疗方法，均实现了轴突切断、套管化神经的再生。所分析的远端神经段显示出与正常神经相似的结构。
